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| 改良单克隆抗体Dot—ELISA检测血吸虫循环抗原 | |
| 引用本文： | 娄文娴 薛纯良. 改良单克隆抗体Dot—ELISA检测血吸虫循环抗原[J]. 上海交通大学学报(医学版), 1991, 11(4): 285-288 |
| 作者姓名： | 娄文娴 薛纯良 |
| 作者单位： | 上海第二医科大学寄生虫学教研室(娄文娴，薛纯良)，上海第二医科大学寄生虫学教研室(许静瑛) |
| 基金项目： | 国家“七五”攻关课题基金 |
| 摘 要： | 用抗趋阴极循环抗原单克隆抗体建立的Dot-ELISA检测血吸虫循环抗原显示有高度的敏感性和特异性,极少交叉反应。68份急性血吸虫病患者血清的阳性检出率为98.5%,216份慢性血吸虫病血清的阳性检出率为83.3%。225份正常人血清仅1份阳性。48例血吸虫病治愈者的阴转率为97.9%。实验性血吸虫病家兔治疗后8周,16只治愈的家兔中12只阴转。 |
| 关 键 词： | 血吸虫 循环抗原 单克隆抗体 检测 |
Detection of Schistosoma Antigens by Monoclonal Antibody Dot-ELISA |
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| Lou Wenxian,et al. Detection of Schistosoma Antigens by Monoclonal Antibody Dot-ELISA[J]. Journal of Shanghai Jiaotong University:Medical Science, 1991, 11(4): 285-288 | |
| Authors: | Lou Wenxian et al |
| Affiliation: | Lou Wenxian,et al Department of Parasitology,Shanghai Second Medical University,Shanghai |
| Abstract: | Dot-ELISA using an anti-CCA monoclonal antibody to detect schistosoma antigens showed high sensitivity and specificity in the diagnosis of schistosomiasis with very few cross-reactions, In 68 acute schistosomiasis cases, the positive rote was 98.5% 216 chronic cases gave 83.3% positive; 225 normal individuals showed one positive reaction. Aneativeconversion rate of 97.9% in 48 cured schistosomasis patients was demonstrated, In an animal experiment using 16 infected rabbits, 12 negative in the 8th week post treatment. |
| Keywords: | schistosoma circulating antigen anti-CCA monoclonal antibody |
| 本文献已被 CNKI 维普 等数据库收录！ | |