兔肾近端小管细胞的培养

THE CULTURE OF PROXIMAL TUBULE CELLS OF RABBIT KIDNEY

It is a new approach to investigate the ultrastructure, function and metabolism of each segment of uriniferous tubule using dual environment culture chambers (DECC). In the present study the DECC culture of proximal tubule cells was undertaken with millcell-HA culture insert in the mixed medium of Ham's F12 and Dulbecco's modified Eagle's medium.A monolayer of proximal tubule cells was obtained in the medium mixed by constant bubling of oxygen at 3 weeks after seeding. Transmission electron microscopy revealed the cuboidal cells with microvilli, abundant mitochondria, vacuoles, lysosomes, and irregular plasma membrane infoldings. Also, the monolayer exhibited active metabolism from the release of ~(14)C-I-carbon dioxide and absorption ability of ~(14)C-I-glucose across the cultured monolayer. In addition, the culture procedure has been introduced in detail.