柳叶鬼针草总黄酮对四氯化碳所致小鼠急、慢性肝损伤的保护作用

目的：采用四氯化碳（CCl4）腹腔注射制备小鼠急、慢性肝损伤模型，探讨柳叶鬼针草（TFB）〖JP3〗总黄酮对肝损伤的保护作用及其作用机制。方法:60只ICR小鼠随机分为对照组、模型组、阳性药护肝片（4 g?kg-1）〖JP〗组及TFB高、中、低（120、60和30 mg/kg）剂量组，每组10只。采用腹腔注射不同剂量及不同给药次数给予CCl4 分别制备小鼠急、慢性肝损伤模型，观察肝脏指数、血清丙氨酸氨基转移酶（ALT）、天门冬氨酸转氨酶（AST）、超氧化物歧化酶（SOD）和丙二醛（MDA）水平，以及肝脏组织病理形态学变化。结果:与模型组比较，TFB（120、60和30 mg/kg）及阳性药护肝片（4 g/kg）组小鼠肝脏水肿明显减轻（P<0.05或P<0.01），血清AST及ALT活性明显降低（P<0.05或P<0.01），血清SOD活性升高，MDA含量降低（P<0.05或P<0.01），肝脏组织病理损害明显改善。结论:TFB对CCl4所致小鼠急、慢性肝损伤具有明显保护作用，其作用机制可能与抗氧化损伤有关。

Protective effects of total flavonoids of Bidens bipinnata L. on acute and chronic liver injury induced by carbon tetrachloride in mice

Objective To investigate the protective effects of total flavonoids of Bidens bipinnata L.(TFB)on acute and chronic liver injury in mice induced by carbon tetrachloride and mechanisms.Methods Sixty Wistar rats were randomly divided into control group,model group,Hu-Gan-Pian (4g·kg-1)group and TFB (120,60,30mg·kg-1)groups.The acute and chronic liver injury model in mice were induced by different dosages and administration times of carbon tetrachloride respectively to study the effects of TFB on liver index,hepatic morphological changes and detect the contents of SOD,MDA,ALT and AST in serum.Results Compared with model group,the degree of hepatic edema in TFB (120,60,30mg·kg-1)and Hu-Gan-Pian (4g·kg-1) groups were decreased (P<0.05or P<0.01),the contents of AST,ALT and MDA in serum were decreased (P<0.05or P<0.01),and the contents of SOD were increased (P<0.05or P<0.01).TFB and Hu-Gan-Pian could ameliorate the pathological lesion.Conclusion TFB has protective effects on carbon tetrachloride-induced acute and chronic liver injury in mice,which may be related to inhibition of lipid oxidation.