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| CD4~+CD25~+CD127~(low)识别人外周血CD4~+CD25~+调节性T细胞的优势 | |
| 引用本文： | 王会平,翟志敏,张爱梅,徐修才,李庆,张翠萍,卢娟,沈元元,洪海鸥. CD4~+CD25~+CD127~(low)识别人外周血CD4~+CD25~+调节性T细胞的优势[J]. 中国免疫学杂志, 2008, 24(12) |
| 作者姓名： | 王会平 翟志敏 张爱梅 徐修才 李庆 张翠萍 卢娟 沈元元 洪海鸥 |
| 作者单位： | 1. 安徽医科大学附属省立医院中心实验室及血液科,合肥,230001 2. 安徽省体检中心,合肥,230001 |
| 基金项目： | 国家自然科学基金项目资助 |
| 摘 要： | 目的:探索可用于检测人外周血中CD4+CD25+Treg 细胞的最佳标记物.方法:以 52 名健康人和 47 名非血液系统肿瘤患者为研究对象,采用多色免疫荧光素标记和多参数流式细胞术同时检测外周血中CD4+CD25high、CD4+CD25+FoxP3+和 CD4+CD25+CD127lowT 细胞,并以经典指标CD4+CD25high 和 CD4+CD25+FoxP3+为标准,分析和比较CD4+CD25+CD127low作为识别CD4+CD25+ Treg 细胞的可行性及其优势.结果:健康人和肿瘤患者外周血 CD4+CD25high、CD4+ CD25+FoxP3+和CD4+CD25+CDl27+lowT 细胞占CD4+T 细胞百分比分别为(1.769±O.682)%和(2.958±1.392)%;(2.905±1.772)%和(5.128±2.227)%以及(5.396±1.306)%和(7.175±2.565)%.三者呈相同的趋势,即肿瘤患者组>健康人组,且三者之间呈显著正相关(P<0.05).用两种不同标记法高纯度分选CD4+ CD25high 和 CD4+CD25+CD127lowT 细胞群后,FoxP3 阳性率分别为90.9%和92.7%.结论:CD4+CD25+CD127low 三标记法可以帮助识别 CD4+CD25+Treg 和部分激活的 CD4+T 细胞,提高CD4+CD25+Treg细胞的可检出数量,并且不影响细胞活性度,是反映CD4+CD25+Treg 细胞更理想的指标. |
| 关 键 词： | 调节性T细胞 |
The dominance of CD4~+CD25~+ Treg cells in peripheral blood discriminated by CD4~+CD25~+CD127~(low) |
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| WANG Hui-Ping,ZHAI Zhi-Min,ZHANG Ai-Mei,XU Xiu-Cai,LI Qing,ZHANG Cui-Qing,LU Juan,SHEN Yuan-Yuan,HONG Hai-Ou. The dominance of CD4~+CD25~+ Treg cells in peripheral blood discriminated by CD4~+CD25~+CD127~(low)[J]. Chinese Journal of Immunology, 2008, 24(12) | |
| Authors: | WANG Hui-Ping ZHAI Zhi-Min ZHANG Ai-Mei XU Xiu-Cai LI Qing ZHANG Cui-Qing LU Juan SHEN Yuan-Yuan HONG Hai-Ou |
| Affiliation: | WANG Hui-Ping,ZHAI Zhi-Min,ZHANG Ai-Mei,XU Xiu-Cai,LI Qing,ZHANG Cui-Qing,LU Juan,SHEN Yuan-Yuan,HONG Hai-Ou.Affiliated Anhui Provincial Hospital,Medicial University of Anhui,Hefei 230001,China |
| Abstract: | Objective:To investigate the feasibility and dominance of using CD4+CD25+CD127low to discriminate CD4+CD25+ regulatory T cells in peripheral blood.Methods:Numbers of CD4+CD25high,CD4+CD25+FoxP3+ and CD4+CD25+CD127low was detected with the multi-color detection and multi-parameter flow cytometry,using the peripheral blood from 52 healthy people and 47 cancer patients as samples.The ratio of subpopulations in CD4+CD25+Treg was determined and the correlation among the Treg cells as well as dominance and feasib... |
| Keywords: | CD4 CD25 CD127 FoxP3 |
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