高效液相色谱外标法快速测定苯巴比妥血药浓度

目的:建立高效液相色谱(HPLC)外标法快速测定人血清中苯巴比妥的浓度。方法:血清样品经甲醇沉淀后,以甲醇-水(40∶60,V∶V)为流动相,流速为1.0 mL·min-1,色谱柱为Inertsil ODS-SP(150 mm×4.6 mm,5μm),柱温为30℃,紫外检测波长为214 nm。结果:血清内源性杂质不干扰待测物的测定,苯巴比妥的线性范围为4.5～72.8μg·mL-1,日内、日间精密度(RSD)均<5%,样品稳定性良好。结论:该法灵敏、快速、准确、操作简便、线性范围宽,可用于苯巴比妥的血药浓度监测。

Determination of Phenobarbital in Human Serum by HPLC External Standard Method

OBJECTIVE:To establish a HPLC external standard method for rapid determination of Phenobarbital in human serum.METHODS: Serum samples were precipitated by methanol followed by separation on the column of Inertsil ODS-SP(150 mm×4.6 mm,5 μm) at 30 ℃.The mobile phase consisted of methanol-water(40∶60,V∶V) at a flow rate of 1.0 mL·min-1.The detecting wavelength was set at 214 nm.RESULTS: The intrinsic impurity of plasma did not interfere with the determination of analyte.The linear range of arbamazepine was 4.5-72.8 μg·mL-1.The inter-day and intra-day precisions were less than 5%.The samples showed good stability.CONCLUSION: The established HPLC external standard method is sensitive,fast,accurate and simple with wide linear range and thus it can be applied to the monitoring of serum concentration of Phenobarbital.