Inhibition of tumor growth in vitro and in vivo by fucoxanthin against melanoma B16F10 cells |
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| Authors: | Kil-Nam Kim Ginnae Ahn Soo-Jin Heo Sung-Myung Kang Min-Cheol Kang Hye-Mi Yang Daekyung Kim Seong Woon Roh Se-Kwon Kim Byong-Tae Jeon Pyo-Jam Park Won-Kyo Jung You-Jin Jeon |
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| Institution: | 1. Marine Bio Research Team, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea;2. Laboratory of Veterinary Molecular Pathology and Therapeutics, Tokyo University of Agricultural and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan;3. Global Bioresources Research Center, Korea Institute of Ocean Science & Technology, Ansan 426-744, Republic of Korea;4. Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea;5. Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea;6. Korean Nokyong Research Center, College of Medicine Konkuk University, Chungju 380-701, Republic of Korea;7. Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea;8. Department of Marine Life Science, Chosun University, Gwangju 501-759, Republic of Korea |
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| Abstract: | The present study was designed to evaluate the molecular mechanisms of fucoxanthin against melanoma cell lines (B16F10 cells). Fucoxanthin reduced the proliferation of B16F10 cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G0/G1 phase and apoptosis. Fucoxanthin-induced G0/G1 arrest was associated with a marked decrease in the protein expressions of phosphorylated-Rb (retinoblastoma protein), cyclin D (1 and 2) and cyclin-dependent kinase (CDK) 4 and up-regulation of the protein levels of p15INK4B and p27Kip1. Fucoxanthin-induced apoptosis was accompanied with the down-regulation of the protein levels of Bcl-xL, an inhibitor of apoptosis proteins (IAPs), resulting in a sequential activation of caspase-9, caspase-3, and PARP. Furthermore, the anti-tumor effect of fucoxanthin was assessed in vivo in Balb/c mice. Intraperitoneal administration of fucoxanthin significantly inhibited the growth of tumor mass in B16F10 cells implanted mice. |
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