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Identification and phylogenetic analysis of orf virus from goats in Taiwan |
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| Authors: | Kun-Wei Chan Jen-Wei Lin Shu-Hwae Lee Chih-Jung Liao Meng-Chun Tsai Wei-Li Hsu Min-Liang Wong Hung-Chang Shih |
| Institution: | (1) Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, 402, Taiwan;(2) Animal Health Research Institute, Council of Agriculture, Tamsui, 251, Taiwan;(3) Nantou Animal Diseases Control Center, Natou, 540, Taiwan;(4) Graduate Institute of Veterinary Public Health, College of Veterinary Medicine, National Chung-Hsing University, Taichung, 402, Taiwan;(5) Department of Laboratory Medicine, China Medical University Hospital, Taichung, 404, Taiwan |
| Abstract: | An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan. |
| Keywords: | Orf virus B2L gene Phylogenetic analysis |
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