Effects of Intraventricular Injection of Calmodulin Antagonist （Trifluoperazine） on Pregnancy in Rats

Injection of TFP (a specific antagonist of CaM) into the lateral ventricles of the rat brain on the fourth day of pregnancy causes marked antifertility effect in 83% of the rats (0% in the control group). Examination with the pontamone blue reaction for the implantation elucidated that the antifertitity effect was due to the blockage of implantation of the follicle. Injection of TFP into the lateral ventricles of the brain markedly reduced the concentration of CaM in hypothalamus, ovary and uterus. Serum progesterone was also reduced. However, injection glven on day 7 was ineffective to terminate the pregnancy. Injection given on certain time of pregnancy was able to reduce the cellular CaM content and to cause antifertitity. This finding demonstrated that CaM played an important rote during the course of pregnancy.

Effects of Intraventricular Injection of Calmodulin Antagonist(Trifluoperazine)on Pregnancy in Rats

Injection of TFP(a specific antagonist of CaM)into the lateral ventricles of the ratbrain on thefourth day of pregnancy causes marked antifertility effect in 83%of the rats(0%in the control group).Examination with the pontamone blue reaction for theimplantation elucidated that the antifertility effect was due to the blockage ofimplantation of the follicle.Injection of TFP into the lateral ventricles of the brainmarkedly reduced the concentration of CaM in hypothalamus,ovary and uterus.Serumprogesterone was also reduced.However,injection given on day 7 was ineffective to terminate the pregnancy.Injection given on certain time of pregnancy was able to reduce thecellular CaM content and to cause antifertility.This finding demonstrated that CaMplayed an important role during the course of pregnancy.