| Effects of basic fibroblast growth factor on biological characteristics of osteoblasts |
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| 作者姓名： | 曾晖 杜靖远 郑启新 段德宇 刘勇 熊奡 康斌 刘国平 |
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| 作者单位： | [1]DepartmentofOrthopedics,ShenzhenHospitalAffiliatedtoBeijingUniversity,Shenzhen518036,China [2]DepartmentofOrthopedics,XieheHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China |
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| 摘 要： | Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro. Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF ( 5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 ( TGF-β1 )was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Resu/ts: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β1 mRNA increased significantly, but the intracellular ALP content decreased. Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.
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| 关 键 词： | 基质纤维原细胞生长因子 生物学特性 成骨细胞 转化生长因子 细胞培养 |
Effects of basic fibroblast growth factor on biological characteristics of osteoblasts |
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| ZENG Hui,DU Jing-yuan,ZHENG Qi-xin,DUAN De-yu,LIU Yong
XIONG Ao,KANG Bin J and LIU Guo-ping.Effects of basic fibroblast growth factor on biological characteristics of osteoblasts[J].Chinese Journal of Traumatology(English Edition),2003,6(4):229-233. |
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| Authors: | ZENG Hui DU Jing-yuan ZHENG Qi-xin DUAN De-yu LIU Yong
XIONG Ao KANG Bin J and LIU Guo-ping |
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| Institution: | 1. Department of Orthopedics, Shenzhen Hospital Affiliated to Beijing University, Shenzhen 518036, China
2. Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | OBJECTIVE: To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro. METHODS: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. RESULTS: bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased. CONCLUSIONS: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts. |
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| Keywords: | Osteoblasts Fibroblast growth factor basic Transforming growth factor beta Gene expression |
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