复方三根制剂对MDR细胞株K562/ADR和K562/VCR逆转作用的研究

目的：研究复方中药——复方三根制剂对多药耐药(MDR)细胞株的逆转作用。方法：应用MTT比色法，观察复方三根制剂对MDR细胞的逆转作用。运用流式细胞仪(FCM)，测定其对耐药细胞积聚和外排阿霉素(ADR)的影响，以及对耐药株细胞表达P=gp的影响。结果：复方三根制剂可部分恢复K562／ADR和K562／VCR耐药细胞对ADR的敏感性，而对VCR抗药性的逆转作用不明显；可增加K562／ADR和K562／VCR耐药细胞内ADR的积聚，并对外排ADR有一定影响；可部分下调p-gp的表达。结论：复方三根制剂可部分逆转耐药细胞的抗药性。

The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR

Obsjective\ To observe the reversal effect of Fufang Sangen Mixture(FFSG) in multidrug resistant(MDR)cell lines K562/ADR and K562/VCR.Methods\ We used MTT assay to observe the mixture's reversal effect.Then,we determined the effect of FFSG on intracellular adriamycin(ADR) accumulation,drug efflux and the expression of p\|gp by flowcytometry(FCM).Results\ 10mg/ml,20mg/ml of FFSG can reverse the K562/ADR cell's resistance to adriamycin to some degree and 20mg/ml FFSG can also partly reverse the K562/VCR cell's resistance to adriamycin.10mg/ml,20mg/ml concentration of FFSG can enhance the intracellular adriamycin accumulation in K562/ADR and K562/VCR cell lines.FFSG also has the effect on the efflux of intracellular adriamycin and decreasing the expression of p\|gp in MDR cell lines.Conclusion\ FFSG can partly reverse the resistance of MDR cell lines.