人肝门部胆管癌转移动物模型的建立及生物学特性研究

目的利用人肝门部胆管癌细胞系（FRH-0201）接种裸鼠脾脏，建立肝、肺转移模型。方法将FRH-0201细胞系（120代）接种于7只Balb／c裸小鼠脾脏。出现转移时，将转移瘤行组织病理学及超微结构观察。将转移的肿瘤行细胞培养，再次接种裸鼠脾脏，观察转移成瘤情况。结果脾脏局部成瘤率为100％（7／7），转移瘤发生率14．3％（1／7）。转移瘤细胞再次接种于裸小鼠脾脏，转移发生率100％。转移瘤电镜显示典型恶性细胞特征。转移瘤细胞染色体众数19条，主流范围18～44条。结论该实验所建立的肝门部胆管癌转移瘤，符合恶性肿瘤的特点，与人肝门部胆管癌生物学特性一致。

Establishment and characteristics of metastatic tumor model of hilar cholangiocarcinoma

Abstract:Objective:To establish a novel liver and lung metastatic tumor model in nude mice by splenic inoculation of hilar cholangiocarcinoma cell line. Methods:Human hilar cholangiocarcinoma cell line(FPH-0201 cells) was established and cultured with a fresh human hilar cholangiocarcinoma specimen. The spleen of 7 nude mice was injected with 0.1ml of hilar cholangiocarcinoma cell suspension (1.5×107 cell) .The tumor appearing time, tumor local and metastatic formation rate, histological,morphological and ultrastructure of metastatic tumor cell were observed. The sub-cell line was obtained by cutture of metastatic specimen, and sub-cell line cells were again injected into the lower pole of the spleen and metastatic tumor formation rate was observed again. Results:The overall local tumor formation rate in spleen was 100%, and metastatic tumor formation rate was 14.3%. The sub-cell line cells that were re-inoculated in mice spleen, had a metastatic tumor formation rate of 100%. Morphological observation of metastatic tumor showed the common features of cholangiocarcinoma. Chromosome number showed a variation of 18~44. Conclusions:The hilar cholagiocarcinoma metastatic tumor model that was established in this laboratomy had characteristics compatible with malignant tumor and had all the biological characteristics of human hilar cholagiocarcinoma.