HSP70在经热应激预处理后减少大鼠肝脏缺血再灌注损伤过程中的作用

目的　探讨热应激预处理能够减少肝脏缺血再灌注损伤的机制,以期寻找减轻肝脏缺血再灌注损伤的有效措施。方法　建立大鼠肝脏缺血再灌注损伤模型。将42只大鼠随机分为6组:①正常对照组(N);②槲皮素组(Q):腹腔注射槲皮素(quercetin, 7mg·kg-1 );③肝脏缺血再灌注(ischemi areperfusion,I/R)组(I);④热应激预处理组(H+I):缺血再灌注前16h给予热应激预处理;⑤槲皮素+热应激预处理组(Q+H+I):缺血再灌注前16h先给予槲皮素腹腔注射(7mg·kg-1 )再给予热应激处理;⑥槲皮素+缺血组(Q+I):缺血再灌注前16h给予槲皮素腹腔注射(7mg·kg-1 )。观察以上各组大鼠在肝脏缺血再灌注6h后热休克蛋白70(Heatshockprotein70, HSP70 )的表达,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性及肝脏组织形态学改变。结果　大鼠肝脏HSP70表达由高到低依次为:H+I组、I组、Q+H+I组、I+Q组、Q组、N组;而检测血清ALT、AST含量由高到低依次为:Q+I组、I组、Q+H+I组、H+I组、Q组、N组;肝脏组织形态学的改变显示与上述血中ALT、AST含量变化相对应。结论　HSP70在经热应激预处理后减少肝脏缺血再灌注损伤的过程中起重要的作用。

Effects of HSP70 on hepatic ischemia-reperfusion injury after heat preconditioning in rats

Aim To study the effects of HSP70 on hepatic ischemia-reperfusion injury after heat preconditioning in rats.Method To establish the models of the hepatic ischemia-reperfusion injury. 42 Wistar rats were randomly divided into 6 groups, (each group had 7 rats): normal group(N); quercetin injection group(Q); ischemia-reperfusion group(I); heat preconditioning 16 hours before ischemia-reperfusion group(H I); quercetin injection before heat preconditioning group(Q H I); quercetin injection before ischemia-reperfusion group(Q I).We detected the activity of serum enzyme of ALT,AST and the pathological changes of the liver;The expressions of HSP70 of the rats were observed by Western blotting. Results The expressions of HSP70 from high to low were:group H I,group I,group Q H I,group Q I,group Q,group N; The serum levels of ALT and AST from high to low were: group Q I,group I,group Q H I,group H I,group Q,group N;All groups had visibly hepatic histological changes respectively.Conclusion The protection of heat stress pretreatment from ischemia reperfusion injury was possibly performed by inducing the expression of HSP70.