LPS诱导宫颈癌上皮间质转化的作用

目的:探讨LPS在宫颈癌细胞株Hela细胞增殖和上皮间质转化中的作用。方法:体外培养Hela细胞，以LPS(10μg/ml)诱导后，利用MTT法检测细胞的增殖情况。qPCR检测上皮细胞标志物E-cadherin和间质细胞标志物N-cadherin、Vimentin的RNA水平上的表达。Western blot检测上皮细胞标志物E-cadherin和间质细胞标志物N-cadherin、Vimentin的蛋白水平上的表达。Transwell检测LPS对Hela细胞迁移能力的影响。结果:在LPS诱导后能够明显的促进Hela细胞的增殖。E-cadherin在RNA和蛋白水平上都明显下降，N-cadherin、Vimentin在RNA和蛋白水平上都明显升高;LPS诱导后，明显促进Hela细胞的迁移能力。结论:LPS能够诱导宫颈癌细胞株Hela细胞的增殖和上皮间质转化。

LPS induced epithelial-mesenchymal transition of human cervical cancer

Objective:To investigate the effect of LPS on proliferation and epithelial-mesenchymal transition(EMT）of human cervical cancer.Methods:A549 cells cultured in vitro were treated by LPS（10μg/ml).The effect of LPS on proliferation was detected by MTT.The RNA level effect of LPS on epithelial markers(E-cadherin）and mesenchymal markers (N-cadherin，Vimentin) was detected by qPCR.The protein level of LPS on epithelial markers (E-cadherin）and mesenchymal markers (N-cadherin，Vimentin）was detected by Western blot.The effect of LPS on migration of Hela cell was detected by Transwell assay.Results:After treated by LPS，significantly promote the proliferation of Hela cell.The RNA and protein level of epithelial markers(E-cadherin）was decreased，and mesenchymal markers (N-cadherin,Vimentin）was increased.Transwell test showed that the migration potential of Hela cells was significantly increased after LPS treatment.Conclusion:LPS can induce cervical cancer cell line Hela proliferation and epithelial-mesenchymal transition.