| 氯通道阻断剂对NO诱导离体大鼠海马神经元凋亡的保护作用 |
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| 引用本文： | 常全忠,张淑玲,尹金宝,蔡仕宁. 氯通道阻断剂对NO诱导离体大鼠海马神经元凋亡的保护作用[J]. 中国药理学通报, 2009, 25(9) |
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| 作者姓名： | 常全忠 张淑玲 尹金宝 蔡仕宁 |
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| 作者单位： | 遵义医学院珠海校区生理学教研室,广东,珠海,519041 |
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| 基金项目： | 贵州省科技厅资助项目，珠海市科技局资助项目，遵义医学院博士启动基金资助项目 |
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| 摘 要： | 目的观察氯通道阻断剂SITS和DIDS对NO诱导的大鼠离体海马神经元凋亡的保护作用。方法离体培养12d的SD大鼠海马神经元,随机分为正常对照组、NO处理组、NO处理后使用氯通道阻断剂组,对各组神经元分别在相应的时间点进行Hoechst荧光染色观察凋亡细胞数和MTT实验定量检测神经元的存活率,Western blot分析凋亡信号分子Caspase-3的变化。结果SITS和DIDS呈剂量依赖性地抑制NO诱导的神经元损伤,并能抑制损伤所引起的Caspase-3的激活,提高神经元的存活率。结论氯通道阻断剂对NO诱导的大鼠海马神经元凋亡有一定的保护作用。
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| 关 键 词： | 氯通道阻断剂 NO 海马神经元 大鼠 凋亡 Caspase-3 |
The protective effects of chloride channel blockers on the cultured hippocampal neuronal apoptosis induced by NO in rats |
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| CHANG Quan-zhong,ZHANG Shu-ling,YIN Jin-bao,CAI Shi-ning. The protective effects of chloride channel blockers on the cultured hippocampal neuronal apoptosis induced by NO in rats[J]. Chinese Pharmacological Bulletin, 2009, 25(9) |
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| Authors: | CHANG Quan-zhong ZHANG Shu-ling YIN Jin-bao CAI Shi-ning |
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| Abstract: | Aim To observe the protective effects of SITS and DIDS,two kinds of chloride channel blockers,on hippocampal neuronal damage induced by NO in culture.Methods The cultures were divided into three groups:control group,NO treatment group,NO treatment plus chloride channel blocker group. The cultures were detected with the methods of morphological stain (Hoechst 33258),and the apoptotic neurons and neuronal viabilities were observed through MTT quantitative analysis. The activated caspase-3 was analyzed with western blot.Results There were significant protective effects of SITS and DIDS on neuronal damage with dose-dependence.Conclusions Chloride channel blockers have some protective effects against neuronal injury induced by NO. |
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| Keywords: | NO Caspase-3 |
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