笔管草中Megastigmane及黄酮苷类化学成分

目的：研究木贼科植物笔管草(Equisetum debile)的化学成分。方法：采用硅胶、sephadex LH-20凝胶柱色谱进行分离纯化，通过理化方法和光谱分析鉴定化合物结构。结果：从笔管草全草乙酸乙酯和正丁醇提取物中分离得到4个megastigmane型化合物和4个黄酮苷，分别鉴定为blumenol A(1)，corchoinoside C(2)，sannnangaoside A(3)，(3S，5R，6R，7E，9S)-megastigmane-7-ene-3-hydroxy-5，6-epoxy-9-O-β-D-glucopyranoside(4)，山柰酚-3，7-双葡萄糖苷(5)，camelliaside C(6)，山柰酚-3-槐糖苷(7)and clematine(8)．化合物1-4，6，8均为从木贼科植物首次分得。

Megastigmanes and Flavonoid Glycosides of Equisetum debile

AIM: To study the chemical constituents of Equisetum debile. METHODS: Compounds were isolated by column chromatography on silica gel, sephadex LH-20, ODS-18 and their structures were elucidated based on the spectroscopic methods including 2D NMR (COSY, HMQC, HMBC). RESULTS: Four megastigmane type compounds were isolated, one aglycone and three glucosides, identified as blumenol A (1), corchoinoside C (2), sammangaoside A (3), and (3S, 5R, 6R, 7E, 9S)-megastigmane-7-ene-3-hydroxy-5, 6-epoxy-9-O-β-D- glucopyranoside (4). Four flavonoid glycosides, kaempferol-3, 7-di-β-D-glucoside (5), camelliaside C (6), kaempferol 3-O-β-sophoroside (7), clematine (8) were also obtained from this plant. CONCLUSION: All megasigmane compounds and compound 6, 8 were obtained from Equisetaceae family for the first time.