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| 欧白芷素对K562/A02细胞阿霉素耐药的逆转作用(英文) | |
| 引用本文： | 王姝麒,李霞,王小宁,娄红祥. 欧白芷素对K562/A02细胞阿霉素耐药的逆转作用(英文)[J]. 中国天然药物, 2011, 0(2): 146-150 |
| 作者姓名： | 王姝麒 李霞 王小宁 娄红祥 |
| 作者单位： | 山东大学药学院;山东大学海洋学院; |
| 基金项目： | supported by the Natural Science Foundation of Shandong Province (No.Y2008C98)~~ |
| 摘 要： | 目的:探讨欧白芷素对耐药细胞株K562/A02中P-糖蛋白(P-glycoprotein,P-gp)的影响,为抗白血病多药耐药(multi-drug resistance,MDR)提供新方法。方法:采用MTT法观察阿霉素对细胞活力的影响。应用流式细胞术检测欧白芷素对K562和K562/A02细胞内阿霉素累积和细胞中P-gp功能的影响。采用实时定量RT-PCR技术检测MDR1基因在mRNA表达水平的变化。结果:欧白芷素对耐药细胞株K562/A02有显著的逆转耐药活性,最大逆转倍数为7.36。在K562/A02细胞中,欧白芷素明显增加阿霉素的累积,增加了罗丹明123(rhodaminel123,Rh123)蓄积,抑制了Rh123的外排,同时欧白芷素还在mRNA水平抑制了K562/A02细胞中P-gp的表达。结论:欧白芷素能够抑制K562/A02耐药细胞株中MDR1基因表达和P-gp的功能。 |
| 关 键 词： | 欧白芷素 K562/A02细胞 P-糖蛋白 多药耐药 |
Reversal of P-glycoprotein-Mediated Multidrug Resistance in Human Leukemia Cell Line K562/A02 by Archangelicin |
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| WANG Shu-Qi,LI Xia,,WANG Xiao-Ning,LOU Hong-Xiang School of Pharmaceutical Sciences,Sh,ong University,Jinan ,China,Marine College,Sh,ong University at Weihai,Weihai. Reversal of P-glycoprotein-Mediated Multidrug Resistance in Human Leukemia Cell Line K562/A02 by Archangelicin[J]. Chinese JOurnal of Natural Medicines, 2011, 0(2): 146-150 | |
| Authors: | WANG Shu-Qi LI Xia WANG Xiao-Ning LOU Hong-Xiang School of Pharmaceutical Sciences Sh ong University Jinan China Marine College Sh ong University at Weihai Weihai |
| Affiliation: | WANG Shu-Qi1,LI Xia1,2,WANG Xiao-Ning1,LOU Hong-Xiang1 1School of Pharmaceutical Sciences,Shandong University,Jinan 250012,China,2Marine College,Shandong University at Weihai,Weihai 264209 |
| Abstract: | AIM: To investigate the reversal effect of archangelicin (Arc) on multidrug resistance (MDR) in the doxorubicin (Dox)-resistant leukemic cell line K562/A02. METHODS: Cytotoxicity was assayed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method; the DOX and Rh123 concentration in cells and the expression of P-glycoprotein were assayed by flow cytometry. The influence of Arc on P-gp expression at mRNA levels was determined by using real-time RT-PCR. RESULTS: Arc was endowed with remarkable... |
| Keywords: | Archangelicin K562/A02 cells P-glycoprotein Multidrug resistance |
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