| 黄芩素对大鼠心肌缺血和乳鼠心肌细胞损伤的影响(英文) |
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| 引用本文： | 彭军,许文杰,贺碧霞,刘艳,张红,丁启龙. 黄芩素对大鼠心肌缺血和乳鼠心肌细胞损伤的影响(英文)[J]. 中国天然药物, 2011, 0(2): 132-140 |
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| 作者姓名： | 彭军 许文杰 贺碧霞 刘艳 张红 丁启龙 |
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| 作者单位： | 中国药科大学药学医学基础实验教学中心; |
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| 摘 要： | 目的:研究黄芩素对冠脉结扎引起的大鼠心肌缺血损伤及H2O2诱导的乳鼠心肌细胞氧化损伤或Na2S2O4诱导的缺氧/复氧损伤的影响和相关机制。方法:连续灌胃给予大鼠黄芩素7d,最后一次给药后进行冠脉结扎手术,记录结扎前后的心电图,6h后处死大鼠,TTC染色法评估心肌梗死比重,并测定血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量。体外实验中乳鼠心肌细胞与黄芩素共培养3d后用H2O2诱导氧化损伤或用Na2S2O4诱导的缺氧/复氧损伤,MTT法测定细胞活力并测定培养液上清LDH活性。结果:与假手术组相比,冠脉结扎后大鼠心肌梗死率、血清CK、LDH活性和MDA含量明显增加,SOD活性则显著下降;H2O2及缺氧/复氧可造成心肌细胞活力的下降和LDH的释放。与模型组相比,黄芩素的预治疗能有效地抑制缺血引起的大鼠J点抬高,显著降低心肌梗死率,减少血清中CK、LDH活性及MDA含量,但剂量依赖性地降低血清SOD活性。黄芩素的预适应亦可明显地抑制H2O2诱导的心肌细胞活力下降和LDH释放;而对于缺氧/复氧损伤,缺氧时黄芩素的存在与否可影响其心肌保护作用。结论:黄芩素对体内缺血或...
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| 关 键 词： | 黄芩素 心肌缺血 心肌细胞 氧化损伤 ROS |
Effects of Baicalein on Rat Myocardial Ischemia and Neonatal Cardiomyocyte Injury |
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| PENG Jun,XU Wen-Jie,HE Bi-Xia,LIU Yan,ZHANG Hong,DING Qi-Long Experiment Teaching Center of Medical Basis for Pharmacy,China Pharmaceutical University,Nanjing ,China. Effects of Baicalein on Rat Myocardial Ischemia and Neonatal Cardiomyocyte Injury[J]. Chinese JOurnal of Natural Medicines, 2011, 0(2): 132-140 |
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| Authors: | PENG Jun XU Wen-Jie HE Bi-Xia LIU Yan ZHANG Hong DING Qi-Long Experiment Teaching Center of Medical Basis for Pharmacy China Pharmaceutical University Nanjing China |
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| Affiliation: | PENG Jun,XU Wen-Jie,HE Bi-Xia,LIU Yan,ZHANG Hong,DING Qi-Long Experiment Teaching Center of Medical Basis for Pharmacy,China Pharmaceutical University,Nanjing 211198,China |
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| Abstract: | AIM: To investigate the effects and mechanisms of baicalein on myocardial ischemic injury in rats caused by coronary artery ligation, and on H2O2-induced oxidation lesion or Na2S2O4-induced hypoxia/reoxygenation(H/R) injury of neonaltal cardiomyocytes. METHODS: Rats were pretreated with intragastric administration of baicalein for 7 days. After the last administration, animals received the ligation of the left coronary artery (LCA). Electrocardiogram was recorded during the whole ischemic procedure. 6 h aft... |
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| Keywords: | Baicalein Myocardial ischemia Cardiomyocytes Oxidative injury ROS |
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