广西桂林地区淋病奈瑟菌耐药检测结果分析

目的 分析广西桂林地区淋病奈瑟菌(Neisseria gonorrhoeae, NG)对七种抗生素的耐药性,为临床合理用药提供实验室依据。 方法 采用纸片酸度法检测产青霉素酶淋病奈瑟菌(penicillinase-producing Neisseria gonorrhoeae,PPNG),运用琼脂稀释法测抗生素的最小抑菌浓度(minimum inhibitory concentration, MIC)。 结果 从淋病患者标本中分离菌株361株,其中高度耐四环素淋球菌(tetracycline-resistant Neisseria gonorrhoeae, TRNG)和青霉素耐药并PPNG分别为112株(60.22%)和146株(54.89%)。青霉素敏感0株(0.00%),环丙沙星敏感1株(0.28%),四环素敏感28株(7.76%),阿奇霉素敏感236株(65.37%),未发现大观霉素耐药菌株;头孢曲松和头孢克肟敏感分别为341株(94.46%)和354株(98.06%),其非敏感分别为20株(5.54%)和7株(1.94%)。 结论 大观霉素、头孢曲松和头孢克肟可作为桂林地区治疗淋病的首选药物;持续监测NG耐药性对于指导临床合理用药和发现耐药变化具有十分重要的意义。

Detection results of antibiotic resistance of Nesisseria gonorrhoeaestrains isolated in Guilin,Guangxi

Objective To analyze the antibiotic resistance of Neisseria gonorrhoeae (NG) to seven antibiotics in Guilin, Guangxi, and to provide a laboratory basis for rational use of antibiotics in clinical practice. Methods Penicillinase-producing Neisseria gonorrhoeae(PPNG)strains were detected by paper acidimetric method, and minimum inhibitory concentration (MIC) of the antibiotics were determined by the agar dilution method. Results A total of 361 NG strains were isolated from patients with gonorrhea, of which there were 112 strains of high-level tetracycline-resistant Neisseria gonorrhoeae (TRNG) (60.22%) and 146 strains of penicillin-resistant PPNG (54.89%). None of all strains (0.00%) was sensitive to penicillin, and 1 (0.28%) to ciprofloxacin. 28 (7.76%) strains were sensitive to tetracycline, and 236 (65.37%) to azithromycin. No spectinomycin-resistant strains were found. 341 (94.46%) strains were sensitive to ceftriaxone, but 20 (5.54%) strains were non-sensitive to ceftriaxone. 354 (98.06%) strains were sensitive to cefixime, but 7 (1.94%) strains were non-sensitive to cefixime. Conclusion Spectinomycin, ceftriaxone and cefixime can serve as the preferred medication for the treatment of gonorrhea in Guilin. Continuous monitoring of NG resistance is of great significance for guiding rational use of antibiotics in clinical practice and discovering the changes in drug resistance.