冷诱导RNA结合蛋白在放射性肺损伤模型中的表达变化

目的 探讨冷诱导RNA结合蛋白（CIRBP）在放射性肺损伤模型中的表达变化。方法 将30只雄性C57BL/6小鼠按体重随机分为2组，每组15只，对照组小鼠不做任何处理，模型组小鼠经20 Gy X射线单次胸部照射，构建放射性肺损伤模型，于照射后5周解剖。采用苏木素-伊红（H&E）染色和Masson染色观察肺组织病理改变及胶原的沉积；采用免疫组织化学法检测肺组织炎症因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达；采用qRT-PCR技术检测肺组织中CIRBP mRNA的表达；采用免疫荧光技术和Western blotting技术检测肺组织中CIRBP蛋白的表达。结果 与对照组相比，模型组肺组织血管扩张充血、炎细胞浸润、部分肺泡间隔增厚，IL-6的表达（187.22 ±34.77) vs (129.41 ±5.58)，t = 3.179，P < 0.05]和TNF-α的表达（187.02 ±19.16 )vs (137.52 ±23.53)，t = 5.069，P < 0.05]均升高，差异具有统计学意义，而且模型组肺组织中CIRBP mRNA的表达明显升高（1.97 ±0.39) vs (1 ±0.08)，t = 3.45，P < 0.05]。除此之外，免疫荧光和Western blot结果显示模型组CIRBP蛋白表达均明显升高（14.76 ±1.61) vs (9.32 ±1.26)，t = 3.751，P < 0.05；(1.49 ±0.14) vs (1.13 ±0.17)，t = 2.819，P < 0.05]，差异具有统计学意义。结论 CIRBP在放射性肺损伤模型中的表达明显升高，其可能是放射性肺损伤过程中的重要促炎因子。

Expression of cold-inducible RNA-binding protein in radiation-induced lung injury model

Objective To investigate the changes in the expression of cold-inducible RNA-binding protein (CIRBP) in a radiation-induced lung injury model. Methods Thirty male C57BL/6 mice were randomly divided by body weight into control group (no intervention) and model group (single chest X-ray irradiation with a dose of 20 Gy to build a radiation-induced lung injury model). The mice were dissected five weeks after irradiation. Hematoxylin-eosin staining and Masson staining were used to observe the pathological changes of the lung tissue and the deposition of collagen fibers. Immunohistochemistry was used to measure the expression of the inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the lung tissue. qRT-PCR was used to measure the expression of CIRBP mRNA in the lung tissue. The expression of CIRBP protein in the lung tissue was determined by the immunofluorescence assay and Western blot. Results Compared with the control group, the model group showed significant pulmonary vascular congestion, significant inflammatory cell infiltration, significant thickening of some alveolar septa, significantly increased IL-6 expression (129.41 ±5.58) vs (187.22 ±34.77), t = 3.179, P < 0.05], significantly increased TNF-α expression (137.52 ±23.53) vs (187.02 ±19.16), t = 5.069, P < 0.05], significantly increased CIRBP mRNA expression (1 ±0.08) vs (1.97 ±0.39), t = 3.45, P < 0.05], and significantly increased CIRBP protein expression (9.32 ±1.26) vs (14.76 ±1.61), t = 3.751, P < 0.05], by the immunofluorescence assay; (1.13 ±0.17) vs (1.49 ±0.14), t = 2.819, P < 0.05], by Western blot). Conclusion The expression of CIRBP is significantly increased in the radiation-induced lung injury model, which may be an important pro-inflammatory factor in radiation-induced lung injury.