白细胞介素9促进日本血吸虫感染小鼠肝星状细胞激活

目的　探讨小鼠感染日本血吸虫后，白细胞介素-9（interleukin-9, IL-9）在小鼠肝星状细胞活化中的作用。方法　采用肝脏原位灌注消化结合密度梯度离心法，分离日本血吸虫感染7周后小鼠原代肝星状细胞（hepatic stellate cells, HSCs）并进行体外培养。将HSCs分为PBS对照组和IL-9刺激组（浓度20 ng/mL）。分别于刺激后48、72 h收集细胞，采用免疫印迹试验检测HSCs中α平滑肌肌动蛋白（α-smooth muscle actin, α-SMA）、Ⅰ型胶原蛋白（typeⅠcollagen, ColⅠ）和Ⅲ型胶原蛋白（type Ⅲ collagen, Col Ⅲ）表达水平。结果　经20 ng/mL IL-9刺激48 h后，IL-9刺激组HSCs中α-SMA （0.87 ± 0.02） vs. （0.69 ± 0.01）；t = 17.39，P < 0.01]、ColⅠ（0.74 ± 0.02） vs. （0.65 ± 0.01）；t = 9.56，P < 0.01]、Col Ⅲ蛋白 （0.94 ± 0.04） vs. （0.75 ± 0.03）；t = 6.15，P < 0.01]表达水平均显著高于PBS对照组；刺激72 h后，IL-9刺激组HSCs中α-SMA蛋白水平显著高于PBS对照组 （0.76 ± 0.02） vs. （0.58 ± 0.02）；t = 12.52，P < 0.01]，但两组间ColⅠ（0.68 ± 0.02） vs. （0.66 ± 0.02）；t = 1.15，P > 0.05]和Col Ⅲ蛋白（0.75 ± 0.01） vs. （0.72 ± 0.02）；t = 2.22，P > 0.05]表达水平差异均无统计学意义。结论　IL-9可促进日本血吸虫感染小鼠HSCs激活。

Interleukin-9 promotes the activation of hepatic stellate cells in mice infected with Schistosoma japonicum

Objective　To investigate the effects of interleukin-9 (IL-9) in the activation of hepatic stellate cells (HSCs) in mice infected with Schistosoma japonicum. Methods　Primary HSCs were isolated from mice 7 weeks post-infection with S. japonicum using the in situ liver perfusion and density gradient centrifugation, and cultured in vitro. HSCs were randomly assigned to the PBS control group and IL-9 stimulation group (stimulation with 20 ng/mL IL-9). HSCs were harvested 48 h and 72 h post-stimulation, and the expression of α-smooth muscle actin (α-SMA), typeⅠcollagen (ColⅠ) and type Ⅲ collagen (Col Ⅲ) was determined in HSCs using Western blotting. Results　Following stimulation with 20 ng/mL IL-9 for 48 h, the expression of α-SMA (0.87 ± 0.02) vs. (0.69 ± 0.01); t = 17.39, P < 0.01], ColⅠ(0.74 ± 0.02) vs. (0.65 ± 0.01); t = 9.56, P < 0.01] and Col Ⅲ (0.94 ± 0.04) vs. (0.75 ± 0.03); t = 6.15, P < 0.01] was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group. Following stimulation with 20 ng/mL IL-9 for 72 h, the expression of α-SMA was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group (0.76 ± 0.02) vs. (0.58 ± 0.02); t = 12.52, P < 0.01]; however, there were no significant differences between the two groups in terms of ColⅠ(0.68 ± 0.02) vs. (0.66 ± 0.02); t = 1.15, P > 0.05] or Col Ⅲ expression (0.75 ± 0.01) vs. (0.72 ± 0.02); t = 2.22, P > 0.05]. Conclusion　IL-9 promotes the activation of HSCs in mice infected with S. japonicum.