YBX1和FOXA1在胃癌中的表达及其临床病理学意义

目的 探究YBX1和FOXA1在胃癌组织中的表达及其与预后的关系。方法 选取131例胃癌患者癌组织及其相应癌旁组织。qRT-PCR和免疫组织化学法检测YBX1、FOXA1在胃癌及癌旁组织中的表达水平；Crammer's V系数表示胃癌组织中YBX1与FOXA1蛋白表达的相关性，Pearson法分析YBX1 mRNA与FOXA1 mRNA相关性；Kaplan-Meier法分析胃癌组织YBX1、FOXA1蛋白表达与患者5年总生存率的关系；单因素及多因素Cox回归分析影响胃癌患者预后的因素。结果 与癌旁组织相比，癌组织中FOXA1水平降低，YBX1水平升高（P<0.05）。胃癌组织中YBX1 mRNA与FOXA1mRNA水平负相关（r=-0.675, P<0.05）。YBX1与FOXA1蛋白表达负相关（Crammer's V=-0.497,P<0.001）。胃癌组织中YBX1、FOXA1蛋白表达与肿瘤分化程度、淋巴结转移、TNM分期有关（P<0.05）。YBX1阴性表达组和FOXA1阳性表达组5年内存活率分别高于YBX1阳性表达组和FOXA1阴性表达组（均P<0.05）。TNM分期、YBX1是胃癌患者死亡的独立危险因素（P<0.05），FOXA1是胃癌患者死亡的保护因素（P<0.05）。结论 胃癌组织中YBX1高表达、FOXA1低表达，二者与胃癌患者疾病进展及预后有密切联系。

Expression of YBX1 and FOXA1 in Gastric Cancer and Their Clinicopathological Significance

Objective To explore the expression levels of YBX1 and FOXA1 in gastric cancer tissues and determine their relationship with prognosis. Methods A total of 131 patients with gastric cancer were studied, and the corresponding adjacent normal tissues of each patient were selected as the control. qRTPCR and immunohistochemistry were used to detect the expression levels of YBX1 and FOXA1 in cancer tissues and adjacent tissues. The correlation between YBX1 and FOXA1 protein expression in gastric cancer tissues was expressed by Crammer’s V coefficient, and the correlation between YBX1 mRNA and FOXA1 mRNA was analyzed by Pearson method. Kaplan-Meier method was used to analyze the relationship between YBX1, FOXA1 protein expression in gastric cancer tissues and the 5-year overall survival rate of patients. Univariate and multivariate Cox regression analyses were used to analyze the factors affecting the prognosis of patients with gastric cancer. Results Compared with paracancerous tissue, the levels of FOXA1 and YBX1 in cancer tissues were lower and higher, respectively (P<0.05). A negative correlation was observed between YBX1 mRNA and FOXA1 mRNA in gastric cancer (r=?0.675, P<0.05). The expression of YBX1 and FOXA1 proteins in gastric cancer tissues was negatively correlated (Crammer’s V=?0.497, P<0.001). The expression of YBX1 and FOXA1 proteins in gastric cancer tissue was related to the degree of differentiation, lymph node metastasis, and TNM staging (P<0.05). The 5-year survival rate of the YBX1 negative expression group and the FOXA1 positive expression group were higher than those of the YBX1 positive expression group, and the FOXA1 negative expression group both P<0.05). TNM staging and YBX1 were independent risk factors for death in patients with gastric cancer (P<0.05), and FOXA1 was a protective factor (P<0.05). Conclusion YBX1 is highly expressed and FOXA1 is lowly expressed in gastric cancer tissues; they are closely related to the disease progression and prognosis of patients with gastric cancer.