miR-1301-3p与甲状腺乳头状癌中央区淋巴结转移的相关性探讨

目的:研究miR-1301-3p在甲状腺乳头状癌（papillary thyroid carcinoma,PTC）中表达的变化，分析miR-1301-3p与PTC中央区淋巴结转移的关系。方法:选取2018年08月至2019年08月在我院诊断为“甲状腺肿瘤”的患者60例，其中PTC 30例。qRT-PCR检测miR-1301-3p在良、恶性肿瘤组织，及PTC术前、术后血清中的表达。分析miR-1301-3p与中央区淋巴结转移的关系。ROC曲线分析miR-1301-3p对甲状腺肿瘤、中央区淋巴结是否转移的诊断价值。结果:miR-1301-3p在PTC组织中表达降低（P＜0.000 1），在PTC患者术后血清中表达上升(P=0.045 5)。超声对甲状腺肿瘤的诊断效能为0.726（CI:0.627~0.810，P＜0.001）；miR-1301-3p对甲状腺肿瘤的诊断效能为0.788（CI:0.695~0.864，P＜0.001）；miR-1301-3p联合超声对甲状腺肿瘤的诊断效能为0.827（CI:0.738~0.895，P＜0.001）。miR-1301-3p在有中央区淋巴结转移的PTC中表达降低。超声对中央区淋巴结是否转移的诊断效能为0.571（CI:0.410~0.723，P=0.033 9）；miR-1301-3p对中央区淋巴结是否转移的诊断效能为0.813（CI:0.662~0.916，P＜0.001）；miR-1301-3p联合超声对中央区淋巴结是否转移的诊断效能为0.872（CI:0.733~0.955），P＜0.001）。结论:miR-1301-3p在PTC组织中表达降低，与中央区淋巴结转移相关；miR-1301-3p可提高超声对甲状腺肿瘤、中央区淋巴结是否转移的诊断效能，可能为PTC潜在的肿瘤标志物。

The correlation between miR-1301-3p and central lymph node metastasis in papillary thyroid carcinoma

Objective:To determine the expression profile of miR-1301-3p in PTC and analyze the relationship between miR-1301-3p expression and central lymph node metastasis of PTC.Methods:60 patients diagnosed as "thyroid tumor" in our hospital from August 2018 to August 2019 were selected,including 30 cases of PTC.qRT-PCR was used to detect the expression of miR-1301-3p in benign,malignant tumor tissues,and preoperative,postoperative serum.The relationship between miR-1301-3p and central lymph node metastasis was analyzed.ROC curve was used to analyze the diagnostic value of miR-1301-3p in thyroid tumor and central lymph node.Results:The expression of miR-1301-3p decreased in PTC（P<0.000 1),and increased in the serum of postoperative（P=0.045 5).The diagnostic efficiency of ultrasound for thyroid tumor was 0.726（CI:0.627~0.810,P<0.001) and miR-1301-3p was 0.788（CI:0.695~0.864,P<0.001).miR-1301-3p combined with ultrasound was 0.827（CI:0.738~0.895,P<0.001).qRT-PCR and TCGA data analysis showed that miR-1301-3p decreased in PTC with central lymph node metastasis.The diagnostic efficiency of ultrasound for central lymph nodes was 0.571（CI:0.410~0.723,P=0.033 9) and miR-1301-3p for central lymph nodes was 0.813（CI:0.662~0.916,P＜0.001).miR-1301-3p combined with ultrasound for central lymph nodes was 0.872（CI:0.733~0.955,P<0.001).Conclusion:miR-1301-3p expression is decreased in PTC,and it is associated with central lymph node metastasis,miR-1301-3p can be used as a tumor marker for PTC diagnosis and preoperative management.