人体肌卫星细胞培养的实验研究

目的 了解人体肌卫星细胞在体外培养条件下的生物学特性。方法 标本取自创伤病例的四肢骨骼肌，消化、提纯后，在生长培养液内培养10天，细胞生长至汇合后分化培养5天。倒置显微镜观察，绘制细胞生长曲线，计算细胞融合率，结蛋白免疫细胞化学方法（ABC）法鉴定肌卫星细胞。结果 细胞消化、提纯后纯度为90％。在生长培养液内肌卫星细胞分裂增生；在分化培养条件下，汇合状态的肌卫星细胞发生融合，形成肌管，分化培养24小时细胞融合率开始明显增加，分化72小时融合率达到高峰。结论 人体肌卫星细胞在适当的培养条件下可以增生、分化，形成肌纤维。结蛋白免疫细胞化学染色是早期鉴定肌卫星细胞的有效方法。

CULTURE OF HUMAN MUSCLE SATELLITE CELL

Objective To investigate the biological characteristics of human muscle satellite cell cultured in vitro. Methods Human muscle satellite cells were obtained from skeletal muscle biopsies of six patients during corrective orthopedic surgery, cultivated in growth medium for ten days, then in differentiation medium for additional five days. Human satellite cells were identified with monoclonal antibody against desmin. Cells were observed under phase contrast microscopy.Results Human muscle satellite cells proliferated in growth medium, and fused to form myotubes in differentiation medium. After 24 hours in differentiation medium, the confluent satellite cells began to fuse actively and achieved the top level at 72 hours.Conclusion Human muscle satellite cell can proliferate and differentiate in appropriate culture condition. Immunocytochemical detection of desmin is the effective early method to determine satellite cell.