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| 体外分离培养昆明小鼠胚胎干细胞最佳胚龄的筛选:孕2.5d与3.5d及4.5d比较 | |
| 引用本文： | 张海锋,单伟,秦书俭. 体外分离培养昆明小鼠胚胎干细胞最佳胚龄的筛选:孕2.5d与3.5d及4.5d比较[J]. 中国组织工程研究与临床康复, 2010, 14(10). DOI: 10.3969/j.issn.1673-8225.2010.10.014 |
| 作者姓名： | 张海锋 单伟 秦书俭 |
| 作者单位： | 1. 徐州医学院解剖教研室,江苏省徐州市,221004 2. 辽宁医学院解剖教研室,辽宁省锦州市,121001 |
| 基金项目： | 辽宁省自然科学基金，徐州医学院课题基金 |
| 摘 要： | 背景:昆明小鼠基因型与人类近似,建立昆明小鼠胚胎干细胞系有利于其转基因动物的获得,但目前对于何时收集胚胎尚无明确报道.目的:探讨体外分离培养昆明小鼠胚胎干细胞的最佳胚龄.方法:分别从孕2.5 d,3.5 d和4.5 d的昆明系母鼠子宫中分离收集胚胎,于显微镜下观察胚胎的形态、贴壁情况、内细胞团形成情况、胚胎干细胞克隆率、胚胎干细胞亚克隆率等,并进行碱性磷酸酶染色.结果与结论:孕2.5 d采集的胚胎多为16细胞期胚胎,孕3.5 d采集的胚胎多为桑椹胚,孕4.5 d采集的胚胎多为囊胚.孕2.5 d胚胎和孕3.5 d胚胎的贴壁率、内细胞团形成率、胚胎干细胞克隆率、胚胎干细胞亚克隆率均无明显差异(P＞0.05);孕4.5 d胚胎上述指标均显著高丁二前两者,更适合作为胚胎干细胞培养、克隆、分离、传代的材料. |
| 关 键 词： | 昆明小鼠 胚龄 内细胞团 胚胎 胚胎干细胞 |
Screening of optimal embryonic time for in vitro separation and culture of Kunming mouse embryonic stem cells:Comparison among 2.5, 3.5,and 4.5 pregnant days |
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| Zhang Hai-feng,Shan Wei,Qin Shu-jian. Screening of optimal embryonic time for in vitro separation and culture of Kunming mouse embryonic stem cells:Comparison among 2.5, 3.5,and 4.5 pregnant days[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2010, 14(10). DOI: 10.3969/j.issn.1673-8225.2010.10.014 | |
| Authors: | Zhang Hai-feng Shan Wei Qin Shu-jian |
| Affiliation: | Zhang Hai-feng1,Shan Wei2,Qin Shu-jian21Department of Anatomy,Xuzhou Medical College,Xuzhou 221004,Jiangsu Province,China,2Department of Anatomy,Liaoning Medical University,Jinzhou 121001,Liaoning Province |
| Abstract: | BACKGROUND: Genotype of Kunming mice was similar to human population, thus an establishment of embryonic stem cell line is beneficial for research of transgenic animal. However, the best time to collect embryo has been less reported yet. OBJECTIVE: To find the best time to collect embryos from Kunming mice. METHODS: The embryos were collected from mother mice of 2.5, 3.5, and 4.5 pregnant days. Microscope was used to evaluate the growth condition of embryos, embryo attaching rate (A/C), inner cell mass (ICM... |
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