阿魏酸哌嗪对单侧输尿管梗阻大鼠肾脏的保护作用

目的观察阿魏酸哌嗪（Piperazine ferulate，PF）对单侧输尿管梗阻（Unilateral ureteral obstruction，UUO）模型大鼠肾小管间质的转化生长因子-β1（Transforming growth factor-β1，TGF-β1）、α-平滑肌肌动蛋白（α-smooth muscle actin，α—SMA）和Ⅰ型胶原（Collagen Ⅰ，ColⅠ）表达水平的影响，初步探讨其对小管间质纤维化的作用机制。方法将24只大鼠分为假手术（Sham—operated rats，SOR）组、UUO组、氯沙坦（Angiotensin Ⅱ receptor blocker，ARB）组和PF组。运用免疫组化技术半定量分析实验第14天各组大鼠肾组织中TGF-β1、α—SMA和ColⅠ的表达水平。结果免疫组化结果表明SOR组TGF-β1、α—SMA和ColⅠ均为少量表达，UUO组大鼠上述表达明显增加；ARB组和PF组TGF-β1、α—SMA和ColⅠ的表达均低于UUO组，但高于SOR组，两治疗组之间差异无统计学意义。结论阿魏酸哌嗪可以降低肾小管间质TGF-β1、α—SMA和ColⅠ的表达水平，延缓肾小管间质纤维化的进程。

Protective effect of piperazine ferulate on rat kidney after unilateral ureteral obstruction

Objective To explore the effect and mechanism of piperazine ferulate on regulating the expression of transforming growth factor-β1 （TGF-β1）, α-smooth muscle actin （α-SMA） and collagen Ⅰ （ColⅠ） in renal tubulointerstitium in the rat model of unilateral ureteral obstruction （UUO） Methods Twenty-four SD rats were randomly divided into sham-operation group, UUO group, angiotensin Ⅱ receptor blocker treatment group, and piperazine ferulate treatment group. The expression levels of TGF-β1,α-SMA and ColⅠ in the rats were determined by immunohistochemistry assay at 14 days of the experiment. Results Immunohistochemistry results showed that there was slight expression of TGF-β1, α-SMA and ColⅠ in sham-operation group, but the expression in UUO group was significantly increased （P〈 0. 05）. The expression of TGF-β1, α-SMA and ColⅠ in angiotensin Ⅱ receptor blocker treatment and piperazine ferulate treatment groups was lower than that in UUO group but higher than that in sham-operation group （P〈0. 05）. No significant difference in the expression levels was found between the two treatment groups（P〉0. 05）. Conclusion Piperazine ferulate can decrease the expression of TGF-β1,α-SMA and ColⅠ, suggesting it can delay the aggravation of renal tubulointerstitial fibrosis.