| 表面等离子体共振传感器技术在检测甲型流感病毒中的应用研究 |
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| 引用本文： | 滕娟,徐云庆,顾大勇,史蕾,刘春晓,赵纯中,杨燕秋,孙秋香,欧青叶,季明辉. 表面等离子体共振传感器技术在检测甲型流感病毒中的应用研究[J]. 中国国境卫生检疫杂志, 2011, 0(3): 145-149 |
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| 作者姓名： | 滕娟 徐云庆 顾大勇 史蕾 刘春晓 赵纯中 杨燕秋 孙秋香 欧青叶 季明辉 |
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| 作者单位： | 中山大学公共卫生学院;深圳国际旅行卫生保健中心; |
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| 基金项目： | 国家自然科学基金项目(30972827); 国家质检总局科研基金项目(2010IK212); 深港创新圈计划(联合资助)项目(ZYB200907090128A); 深圳市科技计划切块项目(HZ0907004) |
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| 摘 要： | 目的 建立一种基于表面等离子体共振传感器技术检测人类甲型流感病毒的方法,并进行初步诊断应用.方法 分别设计基于甲型流感M基因的引物和探针,制备RT-PCR扩增产物,优化并确定基于传感技术的实验条件,建立高灵敏度和高特异度的表面等离子体共振传感器方法.结果 缓冲液的盐离子强度和pH值是影响杂交效果的关键因素,对其优化后标...
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| 关 键 词： | 表面等离子共振传感技术 甲型流感病毒 检测 应用 |
Surface plasmon resonance biosensor for detecting influenza A virus in human |
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| TENG Juan,XU Yun-qing,GU Da-yong,SHI Lei,LIU Chun-xiao,ZHAO Chun-zhong,YANG Yan-qiu,SUN Qiu-xiang,’ OU Qing-ye,JI Ming-hui. Surface plasmon resonance biosensor for detecting influenza A virus in human[J]. Chinese Journal of Frontier Health and Quarantine, 2011, 0(3): 145-149 |
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| Authors: | TENG Juan XU Yun-qing GU Da-yong SHI Lei LIU Chun-xiao ZHAO Chun-zhong YANG Yan-qiu SUN Qiu-xiang ’ OU Qing-ye JI Ming-hui |
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| Affiliation: | Sun Yat-sen University,School of Public Health,Guangzhou,Guangdong 510080,China |
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| Abstract: | Objective To establish a detection method for influenza-A virus based on surface plasmon resonance (SPR) biosensor. Methods Design respectively primers and probe of influenza A virus based on M gene and prepare RT-PCR product. A sensitive and specific SPR method was established and optimized. Results Salt ionic strength and pH of the buffer were key elements that influence hybridization. The standard curve showed that the reflectivity shifts and PCR product concentration had a good linear relationship ( R 2... |
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| Keywords: | Surface plasmon resonance biosensor Influenza A virus Detection Application |
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