| 三种农药对大蒜根尖细胞姐妹染色单体互换频率及微核率的影响 |
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| 引用本文： | 唐历波,刘燕. 三种农药对大蒜根尖细胞姐妹染色单体互换频率及微核率的影响[J]. 环境与健康杂志, 2009, 26(4): 308-310 |
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| 作者姓名： | 唐历波 刘燕 |
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| 作者单位： | 遵义医学院珠海校区生化教研室,广东,珠海,519041;遵义医学院珠海校区生化教研室,广东,珠海,519041 |
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| 摘 要： | 目的探讨辛硫磷、高效氯氟氰菊酯、四聚乙醛3种农药对大蒜根尖细胞的致畸变作用。方法分别用13.65mg/L的辛硫磷、25mg/L的高效氯氟氰菊酯、25mg/L的四聚乙醛,2%的环磷酰胺CP(阳性对照组)浸泡大蒜根尖24h,阴性对照组置蒸馏水中培养,统计大蒜根尖细胞姐妹染色单体互换(SCE)和微核(MCN)频率。结果辛硫磷组和四聚乙醛组的SCE频率均显著大于阴性对照组(P<0.01),其中辛硫磷SCE显著大于阳性对照(P<0.01),为最高值;高效氯氟氰菊酯组与阴性对照比较差异无统计学意义(P>0.05)。3种农药的MCN频率均高于阳性对照组(P<0.01),四聚乙醛大于阳性对照组(P>0.05)为最高值。结论3种农药中辛硫磷、四聚乙醛均对大蒜根尖细胞有较强的染色体致畸变作用,高效氯氟氰菊酯的诱变性较小。可利用植物根尖细胞检测环境诱变剂。
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| 关 键 词： | 农药 大蒜根尖细胞 姐妹染色单体互换 微核 |
Effect of Pesticides on Sister-Chromatid Exchange and Micronuclei Frequency of Garlic Root Tip Cells |
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| TANG Li-bo,LIU Yan. Effect of Pesticides on Sister-Chromatid Exchange and Micronuclei Frequency of Garlic Root Tip Cells[J]. Journal of Environment and Health, 2009, 26(4): 308-310 |
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| Authors: | TANG Li-bo LIU Yan |
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| Affiliation: | TANG Li-bo,LIU Yan. Zhuhai Campus,Zunyi Medical College,Zhuhai,Guangdong 519043,China |
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| Abstract: | Objective The effect of the pesticides on sister-chromatid exchange and micronuclei frequency of garlic root tip cells was researched. Methods The garlic root tips were treated with 13.65 mg/L phoxim, 25 mg/L lambda-cyhalothrin respectrely.With the positive and negative control, the garlic root tips were treated with 2%CP and distilled water respectively. Sister-chromatid exchange(SCE) and micronucle(iMCN)frequencies were calculated. Results As for the SCE frequency,lambda-cyhalothrin was the same as the ne... |
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| Keywords: | Pesticides Garlic root tip cells Sister-chromatid exchange Micronuclei |
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