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| 肌肉注射脂联素基因对高脂饮食大鼠氧化应激作用的影响 | |
| 引用本文： | 范颖,苏本利,孟秀香,刘丹丹,刘奔. 肌肉注射脂联素基因对高脂饮食大鼠氧化应激作用的影响[J]. 生物医学工程研究, 2009, 28(2): 116-118,123 |
| 作者姓名： | 范颖 苏本利 孟秀香 刘丹丹 刘奔 |
| 作者单位： | 1. 大连医科大学诊断学试验中心,大连,116045 2. 大连医科大学附属二院内分泌科,大连,116027 |
| 摘 要： | 构建人脂联素(hAPN)基因的质粒表达载体,研究其体外转移后的表达情况.通过基因重组构建脂联素基因表达系统,电转导法导入大鼠股部肌肉,RT-PCR检测大鼠肌肉注射部位组织中mRNA水平的表达情况,同时监测外周血中SOD、MDA水平.结果表明:RT-PCR显示该基因能在肌肉组织中表达,血清中SOD水平相应升高,注射后两周达到最高水平;MDA水平降低,注射两周后达到最低水平,持续大约两周.脂联素表达系统可以在动物体内表达,增加了自由基的清除,抑制了脂质过氧化物的产生. |
| 关 键 词： | 脂联素 氧化应激 超氧化物歧化酶(SOD) 丙二醛(MDA) |
The Effect of Transferred Human Adiponectin Gene on Oxidation Stress of Rats Fed with High-fat Food |
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| FAN Ying,SU Benli,MENG Xiuxiang,LIU Dandan,LIU Ben. The Effect of Transferred Human Adiponectin Gene on Oxidation Stress of Rats Fed with High-fat Food[J]. Journal Of Blomedical Englneerlng Research, 2009, 28(2): 116-118,123 | |
| Authors: | FAN Ying SU Benli MENG Xiuxiang LIU Dandan LIU Ben |
| Affiliation: | 1.Laboratory Center of Diagnosis in Dalian Medical University;Dalian 116045;China;2.Department of Endocrinology;The Second Affiliated Hospital of Dalian Medical University;Dalian 116045 |
| Abstract: | To construct plasmid transferred human APN gene and research human APN expression in rats.Human APN expression system was constructed by gene recombination,transfered plasmid to rat muscle by electrical perforation;mRNA expression was detected by RT-PCR.SOD and MDA level in plasma were monitored.Results showed that mRNA expressed in rat muscle.SOD level ascended after gene injection,and reached the highest after two weeks;MDA level descended after injection,and was the lowest after two weeks.Human APN gene ... |
| Keywords: | Adiponectin Oxidation stress Superoxide dismutase(SOD) Malondialdehyde (MDA) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |