| 福建省登革病毒感染的型别鉴定 |
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| 引用本文： | 翁育伟,李世清,何似,许龙善,严延生. 福建省登革病毒感染的型别鉴定[J]. 海峡预防医学杂志, 2002, 8(6): 12-13 |
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| 作者姓名： | 翁育伟 李世清 何似 许龙善 严延生 |
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| 作者单位： | 福建省流行病学研究所,福州,350001;福建省流行病学研究所,福州,350001;福建省流行病学研究所,福州,350001;福建省流行病学研究所,福州,350001;福建省流行病学研究所,福州,350001 |
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| 基金项目： | 福建省卫生厅科技项目 (项目号 2 0 0 0 - 0 5) |
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| 摘 要： | [目的]应用RT－PCR方法鉴别福建省登革热感染及型别的判断。[方法]从血清中提取病毒RNA,行RTPCR,再用型特异性引物扩增出特异性片段,电泳后观察判断其型别。[结果]从6份早期病人血清扩增出4份登革热2型特异性条带。[结论]该方法可用于登革热感染的鉴别诊断并判断其血清型。
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| 关 键 词： | 登革热病毒 血清型 鉴定 RT-PCR |
| 文章编号： | 1007-2705(2002)06-0012-02 |
| 修稿时间： | 2001-12-13 |
Identification for the Serotype of Dengue Virus Infected in Fujian |
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| WENG Yu wei,LI Shi qing,HE Shi,et al. Identification for the Serotype of Dengue Virus Infected in Fujian[J]. Strait Journal of Preventive Medicine, 2002, 8(6): 12-13 |
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| Authors: | WENG Yu wei LI Shi qing HE Shi et al |
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| Abstract: | To identify the serotype of infection by dengue virus in Fujian, China. The RNA extracted from sera of patient was used to synthesize cDNA in a reverse transcriptase polymerase chain reaction (RT PCR) with a pair of universal primers. The serotype of virus by the sizes of products yielded in a second round PCR amplification with type specific primers was analyzed. The products of 4 from 6 sera specimens were specific fragment of dengue Type 2 virus. [Conclusion] The RT PCR method can be used to identify the infection by dengue virus and the serotype can be typed. |
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| Keywords: | Dengue virus serotype identification reverse transcriptase polymerase chain reaction (RT PCR) |
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