米非司酮对人外周血来源树突状细胞成熟和功能的影响

目的观察米非司酮对人外周血来源树突状细胞(DCs)成熟及生物学功能的影响,探讨米非司酮作为紧急避孕药的免疫学机制。方法人外周血CD14+单核细胞在体外经GM-CSF、IL-4培养6d诱导分化为未成熟DCs,加入1800 nmol/L米非司酮继续培养48h,流式细胞仪检测细胞表型,ELSIA检测DCs培养上清液中IL-12p70水平,混合淋巴细胞反应检测DCs刺激同种异体T细胞增殖的能力。结果与阴性对照组相比,米非司酮处理后的DCs,其细胞表面HLA-DR及CD83分子表达上调(t分别=15.23、7.63,P均<0.05),IL-12p70分泌增加(t=12.62,P<0.05),且刺激同种异体T细胞增殖的能力明显增强,差异均有统计学意义(t分别=9.12、13.24、6.78,P均<0.05)。结论米非司酮能诱导人外周血来源DCs成熟,促进DCs诱导的免疫应答启动。

Mifepristone induces maturation of human monocyte-derived dendritic cells

Objective To investigate the effect of mifepristone on the phenotypic and functional maturation of human moncyte-derived DCs, and consequently, to elucidate the immunological mechanism of mifepristone for emergency contraception. Methods DCs were generated from human peripheral blood CD14^＋ cells cultured with GM-CSF and IL-4. On day 6, immature DCs were stimulated with 1800 nmol/L mifepristone. After 48 hours, DCs were harvested and stained with mAbs against CDla, CD83 and HLA-DR to determine their phenotype by flow cytometric analysis. The cytokine levels secreted by DCs were determined by ELISA and the allostimulatory capacity of DCs was measured by MLR. Results Compared with negative control, mifepristone-treated DCs upregulated the surface expression of MHC class II molecules HLA-DR and specific molecule CD83 （t=15.23, 7.63 ,P〈0.05）, enhanced the secretion of IL-12p70 （t=12.62, P 〈 0.05）and strongly stimulated the proliferation of allogeneic T cell in a mixed lymphocyte reaction （t=9.12,13.24,6.78, P〈0.05）. Conclusions Mifepristone induced a distinct phenotypic and functional maturation of human moncyte-derived DCs, stimulating the immune response induced by DCs.