茶多酚在骨肉瘤顺铂化疗中增效作用的实验研究

目的探讨茶多酚在骨肉瘤细胞株顺铂化疗中的增效作用。方法骨肉瘤细胞株分别经茶多酚、顺铂、茶多酚+顺铂作用72h,采用体外细胞毒性试验观察三者的细胞毒性;应用流式细胞仪分析茶多酚、顺铂及二者配伍对骨肉瘤细胞株的细胞周期的影响。结果骨肉瘤细胞株与浓度为50、100、200、400μg/ml的茶多酚作用时,其细胞毒性指数分别为9.62%、12.30%、28.85%、46.15%,与空白对照组比较差异有统计学意义(F=128.82,P<0.05)。与浓度为0.1、1.0、10.0、100.0μg/ml的顺铂作用时,其细胞毒性指数分别为24.04%、54.80%、65.38%、88.46%,与空格对照组比较,差异有统计学意义(F=75.97,P<0.05)。而低浓度茶多酚与顺铂联合作用,其细胞毒性指数明显增加,随着各自浓度的升高,其细胞毒性作用明显增加。应用流式细胞仪分析显示,茶多酚对细胞周期也有明显的影响,使G0与G1期细胞比例明显增加(F=32.50,P<0.05),G2与M期细胞比例明显减少(F=8.32,P<0.05),S期比例无明显改变。结论茶多酚在骨肉瘤细胞株顺铂化疗中有显著的增效作用,并能改变骨肉瘤细胞的细胞周期。

Enhancement effect of tea polyphenols combined with cisplatin on osteosarcoma S-SLM cell line

Objective To observe the cytotoxicity effect of tea polyphenols combined with cisplatin on osteosarcoma S-SLM cell line.Methods The osteosarcoma S-SLM cell line was treated with different concentration tea polyphenols,cisplatin,tea polyphenols＋cisplatin for 72 hours,respectively,by using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay in vitro,the cytotoxicity effect were observed;By using flow cytometry（FCM）,the cell cycle phases were analyzed.Results Osteosarcoma cell line（S-SLM） was treated with different concentration of tea polyphenols（50,100,200,400 μg/ml）,the cytotoxicity index were 9.62%,12.30%,28.85%,46.15%,compared with the control group,the difference was significant（F=128.82,P0.05）.And treated with different concentration of cisplatin（0.1,1.0,10.0,100.0 μg/ml）,the cytotoxicity index were 24.04%,54.80%,65.38% and 88.46% compared with the space group,the difference was significant（F=75.97,P0.05）.The cytotoxicity index increased obviously as the concentration of tea polyphenols or cisplatin increased.While treated with tea polyphenols and cisplatin simultaneously,the cytotoxicity index increased obviously.The cell cycle of the treated S-SLM cell line was changed with marked increasing in G0/G1 phase fraction and decreasing in G2/M phase fraction（F=32.50,8.32,P0.05）,and S phase fraction did not change significantly.Conclusions Tea polyphenols combined with cisplatin had enhancement cytotoxicity effect on osteosarcoma S-SLM cell line,and also can change the cell cycle of the treated S-SLM cell line.