miR-205-5p、PDCD5、Beclin1在子宫内膜癌组织中的表达及相关性研究

目的 研究子宫内膜癌组织中微小RNA(microRNA miR-205-5p)、程序性细胞坏死因子5（programmed cell necrosis factor 5,PDCD5）、Beclin1的表达情况及三者之间的相关性。 方法 选择75例子宫内膜癌组织和距离癌组织边缘5 cm癌旁组织。通过免疫组织化学、实时荧光定量法检测miR-205-5p、PDCD5、Beclin1的表达情况，分析miR-205-5p、PDCD5、Beclin1的相关性及对子宫内膜癌的诊断价值。 结果 癌组织中miR-205-5p表达高于癌旁组织，PDCD5、Beclin1表达低于癌旁组织（P＜0.05）。不同组织学分级、临床分期、淋巴结转移、肌层浸润子宫内膜癌患者miR-205-5p、PDCD5、Beclin1表达差异有统计学意义（P＜0.05），Ⅲ~Ⅳ期、中低分化、有淋巴结转移、≥1/2肌层浸润子宫内膜癌患者miR-205-5p表达升高，PDCD5、Beclin1表达降低，差异有统计学意义（P＜0.05）。不同年龄、病理类型子宫内膜癌患者miR-205-5p、PDCD5、Beclin1表达差异无统计学意义（P＞0.05）。miR-205-5p、PDCD5之间呈负相关，miR-205-5p、Beclin1之间呈负相关，PDCD5、Beclin1之间呈正相关（P＜0.05）。三项联合诊断子宫内膜癌的价值高于miR-205-5p、PDCD5、Beclin1单项诊断（P＜0.05）。 结论 miR-205-5p表达升高，PDCD5、Beclin1表达降低对子宫内膜癌的发展起到了促进作用，参与子宫内膜癌的演变进程，临床可根据上述指标对子宫内膜癌进行预测。

Expression of miR-205-5p,PDCD5 and Beclin1 in endometrial cancer tissues and their correlation

Objective To explore the expression of microRNA-205-5p(miR-205-5p), programmed cell necrosis factor 5(PDCD5), and Beclin1 in endometrial cancer tissues and their correlation.Methods In total, 75 cases of endometrial cancer tissues and adjacent tissues 5 cm away from the edge of the cancerous tissue of endometrial cancer were selected. The expressions of miR-205-5p, PDCD5, and Beclin1 were detected by immunohistochemistry and real-time fluorescence quantification methods, and the correlation of MiR-205-5p, PDCD5 and Beclin1 and their diagnostic value for endometrial cancer were analyzed.Results The expression of miR-205-5p in cancer tissues was higher than that in adjacent tissues, while the expression of PDCD5 and Beclin1 were lower than that in adjacent tissues(P＜0.05). The expressions of miR-205-5p, PDCD5 and Beclin1 in endometrial cancer patients with different histological grade, clinical stage, lymph node metastasis and muscle invasion were significantly different(P＜0.05). The expressions of miR-205-5p were increased in endometrial cancer patients with stage Ⅲ-Ⅳ, moderate-to-low differentiation, lymph node metastasis and muscle invasion≥1/2 , while the expression of PDCD5 and Beclin1 were decreased, and the difference was statistically significant(P＜0.05). There were no significant differences in the expressions of miR-205-5p, PDCD5 and Beclin1 in endometrial cancer patients with different ages and pathological types(P＞0.05). There was negative correlation between miR-205-5p and PDCD5, negative correlation between miR-205-5p and Beclin1, and positive correlation between PDCD5 and Beclin1(P＜0.05). The value of combined detection of the three in the diagnosis of endometrial cancer was higher than that of miR-205-5p, PDCD5 and Beclin1 alone(P＜0.05).Conclusion The increased expression of miR-205-5p and the decreased expression of PDCD5 and Beclin1 promote the development of endometrial cancer and participate in the evolution of endometrial cancer. The endometrial cancer can be predicted in clinical practice according to the above indicators.