Nestin expression and glial response in the hippocampus of mice after trimethyltin treatment |
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| Affiliation: | 1. College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea;2. Department of Physiology and Neuroscience Program, Michigan State University, MI 48824, USA;3. College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea;1. Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey;2. Department of Biophysics, Faculty of Medicine, Akdeniz University, Antalya, Turkey;3. Department of Physiology, Faculty of Medicine, Near East University, Nicosia, Mersin 10, Turkey;1. Cerebrovascular Diseases Research Institute and Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing, China;2. Beijing Geriatric Medical Research Center and Beijing Key Laboratory of Translational Medicine for Cerebrovascular Diseases, Beijing, China;3. Beijing Institute for Brain Disorders, Beijing, China;1. Departamento de Bioquímica, Programa de Pós-Graduação em Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;2. Programa de Pós-Graduação em Neurociência, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil;3. Laboratório Experimental de Doenças Neurodegenerativas, Departamento de Farmacologia, Centro de Ciências Biológicas, UFSC, Florianópolis, SC, Brazil;1. Animal Science Postgraduate Program, Universidade Federal Rural do Semi-Árido, Mossoró, RN 59600-971, Brazil;2. Universidade Federal Rural do Semi-Árido, Medicine Veterinary Graduation Course, Mossoró, RN 59600-971, Brazil;3. Federal University of Rio Grande do Norte, Department of Biochemistry, Natal, RN 59072-940, Brazil;4. University of São Paulo, School of Veterinary Medicine and Animal Science, Department of Surgery, São Paulo, SP 05508-270, Brazil;1. Department of Neurology and Norman Fixel Institute of Neurological Diseases, College of Medicine, University of Florida, Gainesville, FL, USA;2. Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu, PR China |
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| Abstract: | Nestin is a protein of embryonic intermediate filaments expressed by multipotent neural stem cells. In the present study, the nestin expression pattern in the mouse hippocampus 1, 2, 3, 4, and 8 days after treatment with trimethyltin (TMT) was examined to explore the possible role played by nestin in chemically induced hippocampal injury. TMT treatment (2.5 mg/kg, intraperitoneally) selectively injured the dentate gyrus (DG) of the mouse hippocampus. The level of hippocampal mRNA encoding nestin increased significantly 2 and 3 days post-treatment and thereafter decreased (at 4 and 8 days post-treatment). The level of nestin protein significantly increased 2 – 4 days post-treatment, particularly in the injured region of the DG, and predominantly in glial fibrillary acidic protein-positive astrocytes in the hippocampal DG. Ki67-positive proliferating cells were increased following TMT treatment and co-localized with nestin-positive reactive astrocytes. Thus, we suggest that nestin contributes to remodeling of the chemically injured DG via glial scar formation and the alteration of neurogenesis. |
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| Keywords: | Nestin Hippocampus Trimethyltin Dentate gyrus Astrocytes Mice |
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