新型酞菁类光敏剂的合成及其在光动力学抗菌化学疗法中的应用

目的 发展具有良好溶解性的酞菁类光敏剂,为其应用于光动力抗菌化学疗法（photodynamic antimicrobial chemotherapy,PACT）灭活耐药细菌提供指导依据。方法 通过有机合成的方法合成了4个酞菁化合物（6-9）,并使用元素分析、紫外可见光谱和高分辨质谱等方法表征其结构,进而对其进行PACT和最小抑菌浓度（minimum inhibitory concentration,MIC）实验。结果 成功合成了目标化合物6-9;MIC实验结果表明,化合物6-9对测试的3种耐药菌均无灭活作用;PACT实验结果表明,化合物6-9分别对3种耐药菌有一定程度的灭活作用,其中化合物6灭菌活性最佳,且随着浓度的升高,光动力灭菌效果增强。结论 酞菁化合物是一类优秀的光敏剂,通过增加酞菁化合物的溶解度可有效提高其光动力灭菌效果。

The synthesis of new phthalocyanines as photosensitizer and application in photodynamic antibacterial chemotherapy

Objective Developing the phthalocyanines with good solubility as photosensitizer and providing the guidance for their application in photodynamic antimicrobial chemotherapy （PACT） to inactivate drug-resistant bacteria. Methods Four phthalocyanine compounds （6-9） were synthesized by organic synthesis, their structures were characterized by elemental analysis and UV-Vis spectroscopy, and PACT and minimum inhibitory concentration （MIC） experiments were carried out on them. Results The target compounds 6-9 was successfully synthesized; MIC results showed that compounds 6-9 had no inactivation effect on the three drug-resistant bacteria tested; PACT data indicated that compounds 6-9 showed a certain degree of activity in PACT, especially for compound 6 which showed the best activity for all three bacteria, and with the increase of its concentration, the photodynamic antibacterial effect is enhanced. Conclusion Phthalocyanine compounds are excellent photosensitizers, and the photodynamic antibacterial effect can be effectively improved by increasing the solubility of phthalocyanine compounds.