诱导建立人胰腺癌耐放射细胞亚株的体外实验研究

目的 探索诱导并建立人胰腺癌耐放射细胞亚株的体外实验方法。方法 应用X射线对人胰腺癌细胞株SW1990、Capan-1、AsPC-1、MIAPaCa-2、PANC-1及P3进行梯度递增的诱导照射,随后观察其细胞形态学、细胞周期及放射敏感性的变化。结果 与亲本株相比,各株细胞受照射后形态均出现明显改变;耐放射株SW1990-R、ASPC—R、MIA-R、PAN—R及P3-R的G2／M均明显降低,而放射敏感性指标SF2升高9％～45％。结论 梯度增加照射剂量是可行的体外诱导建立人胰腺癌耐放射细胞亚株的方法。

The method of inducing and establishing human pancreatic cancer cell sublines with radiation resistance

Objective To explore the method of inducing and building pancreatic cancer cell sublines with radiation resistance. Methods Simulating the clinical radiotherapy, the pancreatic cell lines SW1990, Capan-1(Cap), AsPC-1(ASPC), P3, PANC-1(Pan-1) and MIAPaCa-2(MIA) were repeatedly given individual dose of X-rays with liner accelerator to induce radiation resistance, the changes of cell morphology, cell cycle and radio sensibility in the induced cell lines were compared with the parental cell lines at the end of inducing course.Results Compared with the parental cells, there were significant changes in morphology in the pancreatic cancer cell sublines after the radiation. Cell cycle analysis suggested that SW1990-R, ASPC-R, MIA-R, PAN-R and P3-R had lower G_2/M and greater SF_2 (survival fraction after 2 Gy irradiation) compared with the parental cell lines. Conclusions The method of radiating cells step by step and repeatedly is viable to establish radio-resistant pancreatic cancer cell lines.