| mTOR信号转导通路与三氧化二砷反应关联的研究 |
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| 引用本文： | 姜飞,刘卓刚.mTOR信号转导通路与三氧化二砷反应关联的研究[J].中国实验血液学杂志,2010,18(1):54-56. |
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| 作者姓名： | 姜飞 刘卓刚 |
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| 作者单位： | 中国医科大学附属盛京医院血液科,辽宁沈阳,110001 |
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| 摘 要： | 本研究探讨三氧化二砷(ATO)作用与mTOR信号通路的联系。应用Western blot方法检测K562/DNR细胞经ATO处理不同时间后细胞中pmTOR、pAKT及pP70S6K的表达;应用流式细胞术检测K562/DNR细胞经ATO联合LY294002或雷帕霉素处理120小时后细胞的凋亡率。结果表明:K562/DNR细胞经ATO处理60分钟及120分钟时pmTOR表达高于对照组(p0.01);经ATO处理30分钟及60分钟时pAKT表达高于对照组(p0.01);经ATO处理60分钟时pP70S6K表达高于对照组(p0.01)。K562/DNR细胞经ATO联合LY294002或雷帕霉素处理后细胞凋亡率高于对照组(p0.01)。结论:K562/DNR细胞经一定浓度的ATO处理后细胞中mTOR信号通路被激活;mTOR信号通路抑制剂可增强ATO触发K562/DNR细胞凋亡的作用。
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| 关 键 词： | 信号转导通路 mTOR AKT P70S6K 三氧化二砷 |
Correlation between mTOR Signaling Transduction Pathway and Arsenic Trioxide Response |
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| JIANG Fei,LIU Zhuo-Gang.Correlation between mTOR Signaling Transduction Pathway and Arsenic Trioxide Response[J].Journal of Experimental Hematology,2010,18(1):54-56. |
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| Authors: | JIANG Fei LIU Zhuo-Gang |
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| Institution: | (Department of Hematology, Shengding Hospital, China Medical Univercity, Shenyang 110004, Liaoning Province, China) |
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| Abstract: | This study was aimed to investigate the correlation between mTOR signaling transduction pathway and arsenic trioxide(ATO) effect.The expressions of pmTOR,pAKT and pP70S6K in K562/DNR treated with ATO for different time were detected by Western blot.The apoptosis rate of K562/DNR treated by ATO combined with LY294002 or rapamycin for 120 hours was assayed by flow cytometry.The results showed that the expression of pmTOR in K562/DNR cells treated with ATO for 60 minutes or 120 minutes was higher than that in ... |
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| Keywords: | signal transduction pathway mTOR AKT P70S6K arsenic trioxide |
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