| HPLC测定闭鞘姜属3种植物中甾体皂苷的含量 |
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| 引用本文： | 李忠琼,林瑞超,杭太俊,傅文. HPLC测定闭鞘姜属3种植物中甾体皂苷的含量[J]. 华西药学杂志, 2001, 16(1): 27-28 |
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| 作者姓名： | 李忠琼 林瑞超 杭太俊 傅文 |
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| 作者单位： | 云南省药品检验所,;中国药品生物制品检定所,;中国药科大学, |
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| 摘 要： | 目的 :用HPLC测定了姜科闭鞘姜属莴笋花 (CostusLacerus)、闭鞘姜 (C .speciosus)、光叶闭鞘姜 (C .Tonkinensis)根茎中薯蓣皂苷及纤细薯蓣皂苷的含量。方法 :分析柱为C8柱 ( 5 μm ,4.6mm× 2 5 0mm) ,乙腈 水 ( 39∶6 1)为流动相 ,检测波长 2 0 5nm。结果 :薯蓣皂苷的平均回收率为 94.0 8% ,RSD为 1.2 % ;纤细薯蓣皂苷的平均回收率为 93.70 % ,RSD为 3.7%。结论 :三种闭鞘姜属植物中薯蓣皂苷的含量不等 ,可合理开发应用。
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| 关 键 词： | 高效液相色谱 闭鞘姜属 薯蓣皂苷 纤细薯蓣皂苷 |
| 文章编号： | 1006-0103(2001)01-0027-02 |
| 修稿时间： | 2000-05-01 |
DETERMINATION OF STEROIDAL SAPONIN IN THREE SPECIES OF COSTUS BY HPLC |
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| LI Zhong-qiong,LIN Rui-chao,Hang Tai-jun,FU Wen. DETERMINATION OF STEROIDAL SAPONIN IN THREE SPECIES OF COSTUS BY HPLC[J]. West China Journal of Pharmaceutical Sciences, 2001, 16(1): 27-28 |
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| Authors: | LI Zhong-qiong LIN Rui-chao Hang Tai-jun FU Wen |
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| Abstract: | OBJECTIVE:HPLC was used to determine the contents of dioscin and gracillin in rhizomes of Costus lacerus,C.speciosus,and C.tonkinensis.METHODS:The column was Alltima C8 column(5 μm,4.6 mm×250 mm).The mobile phase consisted of acetonitrile-water(39∶61).The determination wavelength was 205 nm.RESULTS:The contents of dioscin and gracillin were 0.19% and 0.12% for Costus lacerus;0.56% and 0.60% for C.speciosus;0.25% and 0.15% for C.tonkinensis,respectively.The average recovery of dioscin was 94.08%,RSD was 1.2%;and gracillin was 93.70%,RSD was 3.7%(n=5)respectively.CONCLUSION:Three plants of the genus Costus contain different contents of dioscin and gracillin.They should be developed and applied reasonably. |
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| Keywords: | HPLC Costus Dioscin Gracillin |
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