大鼠脑干胶质瘤模型的建立

目的 建立大鼠脑干胶质瘤模型,为脑干胶质瘤的研究提供动物实验平台.方法 将1×10~6个大鼠C6胶质瘤细胞通过立体定向头架注射到大鼠脑桥,种植后2周对大鼠行磁共振扫描观察,然后行灌流固定取脑,HE染色.结果 磁共振检查均发现肿瘤生长,肿瘤为长T1和T2信号,经大鼠舌静脉注射Gd-DTPA信号明显增强.HE染色显示肿瘤为胶质母细胞瘤,呈浸润性生长,瘤内新生血管丰富,假栅栏样坏死明显.结论 大鼠C6胶质瘤细胞株可以建立脑干胶质瘤模型,成瘤率高,重复性好.

Establishment of C6 rat brainstem glioma model

Objective To establish C6 rat hrainstem glioma model to provide a platform of animal experiment for the research of brainstem gliomas. Method 1×10~6 C6 glioma cells were injected stereotactically into rat brainstem (pons) exactly. 2 weeks after implantation, MRI scan was performed to observe the growth of tumors, then the rats with tumors were perfused with 4% paraformaldehyde and the braiustems were sectioned and inspected. The sections were stained by H&E. Results Tumors were found in the brainstems which were confirmed by MRI scan with a low T1 and high T2 signals, and were enhanced obviously. There was no extraeranial growth of tumor. H&E stains confirmed that the tumor was glioblastoma. The growth was invasive,the new vessels were plentiful, and the palisade-like necrosis was obvious. Conclusions C6 rat brainstem glioma model can be established safely, steadily and repeatedly with a high tumorigenic rate, which can be confirmed by pathology and MRI scan.