不同代次成人正常髓核细胞的形态及生长动力学比较

目的比较不同代次成人正常髓核细胞体外培养的形态及生长动力学差异。方法从成人正常髓核组织分离培养髓核细胞，传代后对细胞形态、生长曲线、噻唑蓝（methyl thiazolyl tetrazolium，MTT）摄取等进行比较研究。结果体外培养条件下，传3代之前的细胞形态正常，胞浆丰富；传4代起，部分细胞的形态逐渐向长梭形演化。生长曲线提示传3代之前的髓核细胞持续增殖能力强。MTT测定吸光度值，传1、3代时差异无统计学意义（P〉0．05），传5、7代与传1代相比，差异具有统计学意义（P〈0．05）。结论体外培养条件下，前3代成人正常髓核细胞形态良好，活性高，增殖能力强，适合作为组织工程椎间盘的种子细胞。

Comparison on morphology and growth kinetics of adult normal nucleus pulposus cells in different passages

Objective To investigate the morphology and growth kinetics of different passages cells derived from the adult normal nucleus pulposus.Methods The cells derived from the adult normal nucleus pulposus were cultured in vitro.A comparative study was performed on cell morphology,growth curve and MTT assay after passaged.Results Cultured in vitro,cells before passage 3 had normal morphology and abundant cytoplasm.Some cells evolved to a long-spindle shape gradually from passage 4.Powerful continuous proliferation was revealed by growth curve before passage 3.The difference of optical density of MTT assay at passage 1 and 3 didn't have statistical significance(P>0.05).The difference of passages 5 and 7 had statistical significance compared with passage 1(P<0.05).Conclusion The cells of adult normal nucleus pulposus before passage 3 could act as seed-cells of tissue-engineering intervertebral disc for their normal morphology and powerful proliferation.