左旋精氨酸对兔肺缺血再灌注损伤的保护作用

目的 :观察左旋精氨酸对在体兔肺缺血再灌注损伤的影响。方法 :复制在体兔肺缺血再灌注损伤模型。 30只日本雄性大耳兔 ,随机分为三组 :假手术组 ,缺血再灌注组 (IR组 )和左旋精氨酸 +缺血再灌注组(Larg组 )。对比观察各组血浆SOD活力 ,NO、MDA含量 ,肺组织湿干重比 (W /D) ,肺损伤组织学定量评价指标 (IQA)及光镜和电镜下的组织形态学改变。结果 :缺血再灌注后Larg组血浆NO含量和SOD活力较S组、IR组明显升高 (P <0 .0 1) ,MDA、W/D、IQA较IR组明显降低 (P <0 .0 5 ,P <0 .0 5 ,P <0 .0 1) ;IR组镜下见有肺组织水肿 ,肺泡损伤严重 ,内皮细胞线粒体水肿或空泡化 ,毛细血管内炎性细胞浸润 ;而Larg组肺组织上述改变明显减轻。结论 :在体兔肺缺血前预先用L Arg处理 ,可减轻随后的缺血再灌注损伤 ;其机制之一可能与L Arg抗氧自由基作用有关

Protective effect of L-arginine on pulmonary injury induced by ischemia-reperfusion in rabbits

Objective:To investigate the effect of L arginine on pulmonary injury in vivo following ischemia reperfusion in the rabbits.Methods:Rabbit lung model of ischemia reperfusion injury was constituted in vivo. Thirty male Japanese white rabbits were divided into three groups randomly:sham operation group(group S),ischemia reperfusion group(group IR) and L arginine+ischemia reperfusion group(group Larg). The activity of plasma superoxide dismutase(SOD),content of plasma nitric oxide(NO) and malondialdehyde(MDA),wet to dry ratio of lung tissue weight(W/D) and index of quantitative assessment of histologic lung injury(IQA) were measured respectively,lung tissue was prepared for light microscopic and electron microscopic observation.Results:The activity of SOD and content of NO was significantly higher in group Larg post I/R than that in group S or in group IR(P<0.01);while the MDA,W/D,IQA values were significantly lower in group Larg than that in group IR(P<0.05, P<0.05 and P<0.01);under light or electron microscopic observation,it was found that in group IR lung edema and alveoli injury were occured,the mitochondrias in endotheliocyte were swollen or vesicular,and more inflammatory cells stayed in capillary chamber;but such injury were lessen markedly in group larg.Conclusion:Prophylactic administ ration of L arginine intravenously can prevent ischemia reperfusion injury in rabbit lung in vivo. The role of L arginine against the oxygen free radical might be involved.