养阴生津方对热盛津伤瘀滞证模型家兔血液流变性和凝血因子的影响

目的:研究养阴生津方对热盛津伤瘀滞证模型家兔血液流变性和凝血因子的影响.方法:采用药物造模方法,复制温病热盛津伤瘀滞证家兔模型,观察养阴生津方对模型家兔血液黏度、血沉、红细胞压积、血小板聚集率、凝血酶原时间(prothrombin time,PT)、凝血酶时间(thrombin time,TT)、部分凝血活酶时间(kaolin partial thromboplastin time,KPTT)、纤维蛋白原(fibrinogen,Fg)、血栓素B2(thromboxane B2,TXB2)和6-酮-前列腺素F1α(6-keto-prostaglandin F1α,6-keto-PGF1α)的影响.结果:养阴生津方能降低血液黏度和红细胞压积,抑制血小板聚集,降低Fg含量,对抗PT、TT、KPTT缩短,协调TXB2、6-keto-PGF1α间平衡.结论:养阴生津方能改善血液流变性,调节凝血功能,抑制血栓形成,是温病"滋而能通"治法的药理学基础之一.

Effects of Yangyin Shengjin Decoction on hemorheological parameters and coagulation factors in model rabbits with syndrome of excessive heat consuming body fluid and blood stasis

OBJECTIVE: To investigate the effects of Yangyin Shengjin Decoction (YYSJD) on hemorheological parameters and coagulation factors in model rabbits with syndrome of excessive heat consuming body fluid and blood stasis. METHODS: Rabbit model with syndrome of excessive heat consuming body fluid and blood stasis was produced. The effects of YYSJD on the blood viscosity, erythrocyte sedimentation rate (ESR), hematocrit, platelet aggregation rate, prothrombin time (PT), thrombin time (TT), kaolin partial thromboplastin time (KPTT), fibrinogen (Fg), thromboxane B(2) (TXB(2)), and 6-keto-prostaglandin F(1alpha) (6-keto-PGF(1alpha)) in the model rabbits were observed. RESULTS: YYSJD decreased the whole blood viscosity and hematocrit, inhibited the platelet aggregation, prolonged PT, TT and KPTT, and reduced the content of Fg. It also regulated the balance between TXB(2) and 6-keto-PGF(1alpha). CONCLUSION: YYSJD can promote the blood circulation, adjust the blood agglutinating function, and decrease the formation of thrombus. This is one of the pharmacological mechanisms of the therapeutic method of "nourishing yin to promote blood circulation" in the theory of traditional Chinese medicine for seasonal febrile diseases.