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| 关节炎小鼠关节组织提取物对内皮细胞增殖影响及mVEGF的关系 | |
| 引用本文： | 鲁静,侯平,张宁,马晓春,赵丽娟. 关节炎小鼠关节组织提取物对内皮细胞增殖影响及mVEGF的关系[J]. 中国免疫学杂志, 2002, 18(6): 383-384,387 |
| 作者姓名： | 鲁静 侯平 张宁 马晓春 赵丽娟 |
| 作者单位： | 中国医科大学第一临床学院风湿免疫内科,沈阳,110001 |
| 摘 要： | 目的 :通过Ⅱ型胶原诱导鼠关节炎 (CIA)动物模型的建立 ,研究探讨CIA小鼠关节组织提取物对内皮细胞增殖影响及抗血管内皮生长因子 (VEGF)中和抗体对其抑制影响。方法 :采用完全无血清培养、用3H TaR掺入技术检测CIA小鼠关节组织提取物及抗VEGF中和抗体对体外培养人脐静脉内皮细胞 (HUVEC)增殖影响。结果 :发现在CIA小鼠关节组织提取物及小鼠VEGF(mVEGF)作用下 ,培养的HUVEC增殖明显 ,并被抗VEGF中和抗体所抑制 ,抑制率分别为 :6 9 9%、72 2 %。结论 :CIA关节炎形成早期关节组织内可能含有大量的VEGF ,并以较强的生物活性形式存在。同时提示 ,VEGF刺激大量滑膜细胞增殖、血管新生、滑膜炎形成 ,VEGF可能是CIA形成过程中关键性炎症介质。 |
| 关 键 词： | 关节炎 提取物 内皮细胞 生长因子 |
| 文章编号： | 1000-484X(2002)06-0383-03 |
Effects of aqueous joint extracts during CIA development on the HUVEC proliferation and expression of VEGF |
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| LU Jing,HOU Ping,ZHANG Ning et al. Effects of aqueous joint extracts during CIA development on the HUVEC proliferation and expression of VEGF[J]. Chinese Journal of Immunology, 2002, 18(6): 383-384,387 | |
| Authors: | LU Jing HOU Ping ZHANG Ning et al |
| Affiliation: | LU Jing,HOU Ping,ZHANG Ning et al.Division of Rheumatology,Department of Internal Medicine,China Medical University,Shenyang 110001 |
| Abstract: | Objective:To investigate the effects of extracts of aqueous joint during CIA development on the HUVEC proliferation in vitro and expression of VEGF Methods: 3H TaR incorporation was used to estimate the HUVEC proliferation by extracts of aqueous joint and VEGF, and was used cultured in an absolutely serum free medium Results:Extracts of aqueqous joint during CIA development stimulated proliferation of HUVEC and this proliferation was inhibited by the anti VEGF neutralizing antibody Conclusion:The expression of VEGF in the extracts of the aqueous joint during an early stage of CIA development increased and expressed biologically active |
| Keywords: | Arthritic Extract Endothelial cell Growth factor |
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