CXCR4和VEGF-C在结肠癌组织中的表达及与淋巴结转移之间的关系

目的探讨趋化因子受体(CXCR4)和血管内皮生长因子C(VEGF-C)在人结肠癌组织中的表达及与淋巴结转移之间的关系。方法选择65例结肠癌标本,应用免疫组织化学染色方法,检测CXCR4和VEGF-C在人结肠癌组织中的表达,同时对其与临床病理参数的关系进行统计学分析。结果CXCR4在结肠癌组织中的阳性表达率为43.1%,其中淋巴结转移组表达率为57.9%,无淋巴结转移组表达率为22.2%,差异有非常显著的统计学意义(P<0.01)。VEGF-C在结肠癌组织中的阳性表达率为50.2%,其中淋巴结转移组表达率为68.4%,无淋巴结转移组表达率为25.9%,二者差异非常显著(P<0.01)。而且,CXCR4阳性表达与VEGF-C阳性表达呈显著正相关(P<0.05)。结肠癌CXCR4和VEGF-C的表达水平与肿瘤细胞淋巴结转移密切相关,而与病人的年龄、性别、肿瘤大小、组织学类型、分化程度及肿瘤浸润深度等无相关性(P>0.05)。结论CXCR4和VEGF-C的表达可作为评估结肠癌病人淋巴道转移的一个观测指标。

Correlation of CXCR4 and VEGF-C expressions with lymph node metastasis in human colon cancer

Objective To investigate the relationship of the expressions of chemokine receptor CXCR4 and vascular endothelial growth factor C (VEGF-C) with lymph node metastasis in human colon cancer. Methods Immuohistochemistry was used to detect the expressions of CXCR4 and VEGF-C in 65 samples of human colon cancer. The relationship between CXCR4 and VEGF-C expressions and the clinicpathological diameters was statistically analyzed. Results The positive rate of CXCR4 expression with lymph node metastasis was higher than that without lymph node metastasis (57.9% vs 22.2%, P<0.01). The positive rate of VEGF-C expression with lymph node metastasis was higher than that without lymph node metastasis (68.4% vs 25.9%, P<0.01). The positive expression of CXCR4 was consistent with the positive expression of VEGF-C, and the expression levels of CXCR4 and VEGF-C were closely correlated with lymph node metastasis, but not with age, sex, tumor size, histological type, cellular differentiation and invasion depth. Conclusion CXCR4 and VEGF-C expressions may become predictors for the lymphatic metastasis of colon cancer.