| 一株新的小单孢菌C-3509抗肿瘤活性成分的分离、纯化和结构鉴定 |
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| 引用本文： | 赵春燕,张文军,高瑞娟,金莲舫,李电东,邵荣光.一株新的小单孢菌C-3509抗肿瘤活性成分的分离、纯化和结构鉴定[J].中国新药杂志,2010(7). |
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| 作者姓名： | 赵春燕 张文军 高瑞娟 金莲舫 李电东 邵荣光 |
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| 作者单位： | 中国医学科学院北京协和医学院医药生物技术研究所; |
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| 基金项目： | 国家重大新药创制专项(2009ZX09301-003); 国家自然科学基金(30772583); 国家973项目(2009CB521807); 国家高技术研究发展计划(863)基金(2006AA02A255) |
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| 摘 要： | 目的:分离纯化小单孢菌C-3509中的抗肿瘤活性成分,并阐明其结构特征。方法:采用多种层析法进行C-3509抗肿瘤活性成分的分离纯化;通过紫外扫描、高分辨电喷雾质谱和核磁共振氢谱揭示其结构特征;利用DNA断裂法检测其抗肿瘤活性。结果:通过对C-3609菌株发酵和活性检测,分离、纯化得到了抗肿瘤活性成分C-3509B;进一步对C-3509B组分进行初步光谱分析与结构鉴定,结果显示该组分结构特征与抗肿瘤抗生素calicheamicinγ1I一致。结论:C-3509B可能是十元环烯二炔calicheamicins的γ1I组分。
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| 关 键 词： | 小单孢菌C-3509 烯二炔 抗肿瘤抗生素 calicheamicin |
Separation,purification and structure identification of the antitumor antibiotic component from fermentation broth of a new micromonospora C-3509 |
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| ZHAO Chun-yan,ZHANG Wen-jun,GAO Rui-juan,JIN Lian-fang,LI Dian-dong,SHAO Rong-guang.Separation,purification and structure identification of the antitumor antibiotic component from fermentation broth of a new micromonospora C-3509[J].Chinese Journal of New Drugs,2010(7). |
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| Authors: | ZHAO Chun-yan ZHANG Wen-jun GAO Rui-juan JIN Lian-fang LI Dian-dong SHAO Rong-guang |
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| Institution: | ZHAO Chun-yan,ZHANG Wen-jun,GAO Rui-juan,JIN Lian-fang,LI Dian-dong,SHAO Rong-guang(Institute of Medicinal Biotechnology,Chinese Academy of Medicinal Sciences , Peking Union Medical College,Beijing 100050,China) |
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| Abstract: | Objective:To identify the antitumor component extracted from the fermentation broth of a new micromonospora C-3509.Methods:Many kinds of chromatography were used to separate and purify the antitumor component.Then,the identification of the active antitumor component was performed by ultraviolet (UV) scanning,high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and proton nuclear magnetic resonance spectorscopy (1H-NMR).Finally,its antitumor effect was analyzed by DNA damage.Results:The anti... |
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| Keywords: | micromonospora C-3509 enediyne antitumor antibiotic calicheamicin |
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