甲状腺乳头状癌患者肿瘤组织和血清中miR-21表达水平检测及其意义

目的：检测甲状腺乳头状癌（PTC）患者肿瘤组织和血清中miR-21的表达情况，并阐明其临床意义。方法：选择46例PTC患者、35例结节性甲状腺肿患者和18名正常体检者作为研究对象。采用Real-time PCR法检测研究对象甲状腺肿瘤组织（正常人为甲状腺组织）和血清中miR-21表达水平并进行比较。分析PTC患者肿瘤组织和血清中miR-21表达水平与临床病理特征的关联性。结果：PTC患者肿瘤组织和血清中miR-21相对表达水平为2.74±1.53和0.21±0.08，明显高于结节性甲状腺肿患者(0.92±0.81和0.08±0.05)和正常人(0.86±0.39和0.07±0.03) (P<0.05)。PTC患者肿瘤组织和血清中miR-21的表达与腺外侵润、远处转移、淋巴结转移和不同TNM分期有关联(P<0.05)，与性别和肿瘤大小无关联。结论：miR-21在PTC患者肿瘤组织和血清中的表达水平升高，其表达水平与PTC临床病理特征有关联；PTC肿瘤组织和血清中miR-21的检测可能用于PTC临床诊断。

Detection of miR-2 1 expression levels in carcinoma tissue and serum of patients with papillary thyroid carcinoma and its significance

Objective To investigate the expressions of miR-21 in carcinoma tissue and serum of the patients with papillary thyroid carcinoma(PTC),and to clarify its clinical significance.Methods Real-time PCR was used to detect the expression levels of miR-21 in carcinoma tissue and serum of 46 PTC patients,thyroid tissue and serum of 35 cases of nodular goiter and 18 healthy people.The correlations of miR-21 expression level with the clinicopathological features of PTC patients were analyzed.Results The relative expression levels of miR-21 in carcinoma tissue and serum of the PTC patients were 2.74±1.53 and 0.21±0.08,and they were 0.92±0.81and0.08±0.05 in carcinoma tissue and serum of the patients with nodular goiter,0.86±0.39 and 0.07±0.03 in normal papillary thyroid tissue and serum of 18 healthy people.The expression levels of miR-21 in carcinoma tissue and serum of the patients with PTC were higher than those of the patients with nodular goiter and healthy people(P<0.05).The expression level of miR-21 in serum and carcinoma tissue of PTC patients had a positive correlation with the extraglandular invasion,distant metastasis,lymph node metastasis,and TNM stages(P<0.05);and it had no correlation with gender and tumor sizes.Conclusion The expression levels of miR-21 in carcinoma tissue and serum of the PTC patients are increased.The up-regulated expression of serum miR-21 is correlated with the clinicopathological features of PTC patients,which indicates that miR-21 could be used as a potential marker for early detection of PTC.