Assignment of the ǵene for F-type phosphofructokinase to human chromosome 10 by somatic cell hybridization and specific immunoprecipitation |
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Authors: | DOMINIQUE WEIL,DOMINIQUE COTTREAU&dagger ,NGUYEN VAN CONG,RÉ GIS REBOURCET,CHANTAL FOUBERT,MARIE-SYLVIE GROSS,JEAN-CLAUDE DREYFUS&dagger ,AXEL KAHN&dagger |
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Affiliation: | *Unitéde Recherches de Génétique Médicale, INSERM U 12, Professeur J. FRÉZAL, Hôpital des Enfants Malades, 149 rue de Sèvres, 75730 Paris Cedex 15;†Institut de Pathologie Moléculaire, INSERM U 129, Hôpital Cochin, 24 rue du Faubourg St Jacques, 75674 Paris Cedex 14 |
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Abstract: | A method of specific immunoprecipitation of minor isozymes was developed and applied to the detection of human F-type phosphofructokinase in man-rodent somatic cell hybrids. This method allowed us to assign the gene for this newly discovered isozyme of phosphofructokinase to chromosome 10. |
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