Synphilin-1 siRNA对6-OHDA诱导的帕金森病大鼠黑质神经保护作用的研究

目的:观察synphilin-1 siRNA对6-羟多巴胺(6-hydroxydopamine,6-OHDA)诱导的帕金森病(PD)大鼠黑质多巴胺能神经元保护作用,从而探讨synphilin-1siRNA在帕金森病治疗中潜在的应用价值.方法:40只Wistar大鼠随机分成4组,假手术组、模型组、siRNA组、siRNA对照组,每组10只.模型组立体定向将6-OHDA注入左侧黑质制作PD模型,siRNA组、siRNA对照组立体定向将synphilin-1siRNA、阴性对照synphilin-1 siRNA分别注入到各组大鼠的左侧黑质,然后用与第一组相同的方法注入6-OHDA,假手术组注入生理盐水作为对照组,术后2周用阿朴吗啡(APO)(0.5 mg/kg)诱发旋转,进行大鼠行为学检测.术后4周取大鼠的术侧中脑黑质利用免疫组化检测synphilin-1,酪氨酸羟化酶(TH)免疫阳性细胞(ir)的表达.结果:与假手术组比较,模型组synphilin-1-ir阳性细胞表达显著增加,而TH-ir阳性细胞显著减少;与模型组比较,siRNA组synphilin-1-ir阳性细胞表达显著减少,而TH-ir阳性细胞显著增加.结论:Synphilin-1在6-OHDA诱导的PD大鼠黑质神经元损伤机制中起一定作用,synphilin-1 siRNA具有神经保护作用,能为帕金森病的治疗提供新的思路和方法.

The Neuroprotective Effect of Synphilin-1 siRNA on the Substantia Nigra Dopaminergic Neuron in the 6-hydroxydopamine Induced Rat Model of Parkinson's Disease

Objective: To explore the potential value of synphilin-1 siRNA in 6-hydroxydopamine(6-OHDA) induced Parkinson's disease(PD).Methods: Forty healthy male Wistar rats were divided into four groups randomly: the sham operation group, model group,siRNA group,siRNA negative control group,ten rats in each group. In model group: 6-OHDA was stereotaxically injected in the left substantia nigra of rat.siRNA group and siRNA negative control group: Synphilin-1 siRNA and synphilin-1 negative control siRNA were stereotax...