Effects of phosphoric acid on bovine enamel bleached with carbamide peroxide

The aim of this study was to measure the demineralization capacity of 37% phosphoric acid on bovine enamel at different time-points after bleaching with 30% carbamide peroxide. Five, 4 × 4-mm sections were obtained from the enamel of 10 bovine incisors. After applying 30% carbamide peroxide (Vivastyle) for 90 min, specimens were stored in artificial saliva for 0, 24, 72 h, or 7 d and then immersed in 37% phosphoric solution. At 15, 30, 60, 90, and 120 s, 5-ml aliquots were extracted. A control group of specimens was not bleached. Ca²⁺ concentrations were measured by atomic absorption spectrophotometry. A larger amount of Ca²⁺ was extracted from enamel by phosphoric acid after the application of 30% carbamide peroxide. Twenty-four hours after bleaching, significantly more Ca²⁺ was extracted from bleached than from control specimens at all time-points, and this greater susceptibility to the action of the acid persisted for at least 1 wk after bleaching.