抗Dsg—3 EC3—4的单链抗体片段在寻常型天疱疮鼠模型中的作用

目的 探讨抗桥粒芯糖蛋白3(Dsg-3)EC3-4的单链抗体片段(ScFv)在寻常型天疱疮(PV)鼠模型中的作用。方法 在不同时间点。将抗Dsg-3 EC3-4的ScFv通过皮下注射至BALB/c新生鼠,从临床表现、组织病理和免疫荧光三方面评价其对PV鼠模型构建的影响。结果 单独注射抗Dsg-3 EC3-4的ScFv不能在BALB/c新生鼠中诱导出PV的改变,任何时间点注射抗Dsg-3 EC3-4的ScFv都不能阻断PV患者血清对BALB/c新生鼠的致病作用。结论 单独注射抗Dsg-3 EC3-4的ScFv缺乏致病性,也不能阻断PV患者血清对BALB/c新生鼠的致病作用。

The effect of single chain variable fragment antibody to EC3-4 fragment of desmoglein 3 in a mouse model of pemphigus vulgaris

Objective To study the effect of single chain variable fragment (ScFv) antibody to EC3-4 fragment of desmoglein (Dsg) 3 in a mouse model of pemphigus vulgaris.Methods The ScFv an- tibody to EC3-4 fragment of Dsg-3 was injected subcutaneously into neonatal BALB/c mice at different time points;the mice were then evaluated clinically,histopathologically and by direct immunoflorescence exami- nation for the development of lesions.Results When injected alone,the ScFv antibody did not induce the appearance of key clinical features of pemphigus vulgaris.The antibody also did not prevent the develop- ment of pemphigus vulgaris features induced by sera of patients with pemphigus vulgaris,regardless of the time point of injection of ScFv antibody.Conclusion The ScFv antibody to EC3-4 fragment of Dsg-3 lacks pathogenicity in neonatal BALB/c mice,and also could not inhibit the development of lesions induced by sera from patients with pemphigus vulgaris.