清肝调脂饮抗非酒精性脂肪性肝炎大鼠脂质过氧化反应的研究

目的:探讨清肝调脂饮对非酒精性脂肪性肝炎(NASH)大鼠脂质过氧化反应的干预机制.方法:Wistar大鼠70只,除10只作为正常对照组外,余60只建立以高脂饲料(基础饲料88％,猪油10％,胆固醇1.5％,胆盐o.5％)诱导的非酒精性脂肪性肝炎大鼠模型并随机分为5组,除模型组外,造模同时ig清肝调脂饮低、中、高剂量组(4.59,9.18,18.39 g·kg-1)和西利宾胺组(27 mg·kg-1,ig)分别按临床等效剂量的0.5,1,2和1倍剂量药物治疗.实验12周,大鼠全部处死.检测各组大鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),甘油三酯(TG)含量,肝组织匀浆肿瘤坏死因子(TNF-α),丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性；肝组织行CD14,细胞色素P450 2E1( CYP2E1)免疫组化染色,阳性结果作定量分析.结果:与空白对照组比较,模型组ALT(62.57±17.08)U·L-1,TNF-α( 902.00±22.66) pg·mg-1,CYP2E1阳性率(0.88±0.07),SOD(81.78±2.24) U·mg-1各指标均显示异常(P ＜0.05,P＜0.01)；与模型组比较,各用药组均能不同程度的改善以上各指标(P＜0.05,P＜0.01).在降低TG,TN F-α方面,清肝调脂饮高剂量组明显优于西药对照组(P＜0.01)；而对其他各指标的改善,清肝调脂饮各剂量组与西药对照组疗效无显著差异.结论:清肝调脂饮具有降低脂质过氧化反应、提高抗氧化能力的作用,从而减轻NASH肝细胞变性坏死程度.

Effect of Qinggan Tiaozhi Decoction on Lipid Peroxidatic in Rat Nonalcoholic Steatohepatitis

Objective:To observe the effect of Qinggan Tiaozhi decoction on lipid peroxidatic reaction in rat nonalcoholic steatohepatitis(NASH).Method: The NASH model was induced by high fat diet,and the modeling rats were randomly divided into five groups: the model group,the low dose,middle dose and high dose group of Qinggan Tiaozhi decoction and the group of silybin,each group were treated with corresponding 4.59,9.18,18.39 g·kg-1 and 27 mg·kg-1,ig.The indexes of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and triglyceride(TG) in serum were detected,the levels of tumor necrosis factor alpha(TNF-α),malondialdehyde(MDA) and superoxide dismutase(SOD) in liver tissue homogenate were measured,CD14 and cytochromes P450 2E1(CYP2E1) in liver tissue for quantitative analysis were assayed by immunohistochemical stain.Result: The indexes of ALT,TNF-α,CYP2E1 and SOD in model group were changed compared with those in normal group(P<0.05,P<0.01),otherwise they were all improved in treated groups than those in model group(P<0.05,P<0.01).The indexes of TG and TNF-α were improved in high dose group of Qinggan Tiaozhi decoction than that in silybin group(P<0.01),and for other indexes,there were similar effects between Qinggan Tiaozhi decoction and silybin group.Conclusion: Compared with silybin,Qinggan Tiaozhi decoction has better effects on NASH.